Protein Info for PGA1_c15270 in Phaeobacter inhibens DSM 17395

Annotation: alanyl-tRNA synthetase AlaS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01411: tRNA-synt_2c
  • amino acids 6 to 552 (547 residues), 731.4 bits, E=1e-223
• TIGR00344: alanine--tRNA ligase
  • amino acids 6 to 864 (859 residues), 977 bits, E=4.5e-298
• PF07973: tRNA_SAD
  • amino acids 651 to 705 (55 residues), 58 bits, 1.1e-19
• PF02272: DHHA1
  • amino acids 741 to 880 (140 residues), 82.4 bits, E=6.7e-27

Best Hits

Swiss-Prot: 81% identical to SYA_RUEST: Alanine--tRNA ligase (alaS) from Ruegeria sp. (strain TM1040)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 76% identity to rde:RD1_2709)

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

• tRNA charging (19/21 steps found)
• shinorine biosynthesis (3/8 steps found)
• vanchrobactin biosynthesis (2/8 steps found)
• ergotamine biosynthesis (4/12 steps found)
• enterobactin biosynthesis (3/11 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• superpathway of chorismate metabolism (36/59 steps found)
• bacillibactin biosynthesis (2/12 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (2/25 steps found)
• phosphinothricin tripeptide biosynthesis (2/25 steps found)
• corallopyronin A biosynthesis (2/30 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.7

Sequence Analysis Tools

See I7EWP4 at UniProt or InterPro

Protein Sequence (883 amino acids)

>PGA1_c15270 alanyl-tRNA synthetase AlaS (Phaeobacter inhibens DSM 17395)
MPTLNDIRSTFLNYFAKQGHEIVSSSPLVPRNDPTLMFANSGMVQFKNCFTGVETRDYKR
ATTSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKEEAIPFAWDLITKEFGI
DKSRLLTTVYHTDDEAFEIWKKVGVPEDRIIRIDTSDNFWQMGPTGPCGPCTEIFYDHGD
HIWGGPPGSADEDGDRFIEIWNVVFMQNEQFEDGTMKALDMQSIDTGMGLERIGALLQGS
HDNYDTDLFKALIEASADVTNVDPYGDQNVHHRVIADHLRSTSFLMADGVMPSNDGRGYV
LRRIMRRAMRHAHLLGAKDPVMHRLVPALVQQMGAAYPELGRAQPMIEETLHQEETRFKQ
TLDRGLKLLDDELSGLDEGAPLPGAAAFKLYDTFGFPLDLTQDALREKGRTVDTDGFDSA
MAEQKAKARAAWSGSGESADSTIWFDVADQAGTTDFLGYETEAAEGQIMALVVDGAQVAE
AGVGTDVQVVLNQTPFYAESGGQVGDSGEIRVDGGVVRVTDTKKAAGVFIHFGKVESGMI
TKGAAAQLEVDHLRRSAIRANHSATHLLHEALREALGDHVAQRGSLNADDRLRFDFSHNK
ALSGEELRRVAVDVNRFIRQNTVVETRIMTPDDARALGAQALFGEKYGDEVRVVSMGEAA
TGKGQDGNTYSIELCGGTHVRQTGDIGTFVILSDSASSAGVRRIEALTGAEAFAHLDREA
LRMAEVANSLKAQPEEVMDRLKALMDERKALQNEVSQLKQQVAMGGGAGGASETKEVGGK
TFLGQALEGVSGKDLRGLIDAHKQKIGSGVILLIANDGGKVAVAAGVTDDLTAEVSAVDV
LKAAVPAVGGKGGGGRPDMAQGGGKDFAGAEEAIKAAEALLAG