Protein Info for PGA1_c15140 in Phaeobacter inhibens DSM 17395

Annotation: DNA gyrase subunit GyrA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 908 TIGR01063: DNA gyrase, A subunit" amino acids 16 to 886 (871 residues), 1090.9 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 37 to 502 (466 residues), 536.7 bits, see alignment E=4.5e-165 PF03989: DNA_gyraseA_C" amino acids 534 to 581 (48 residues), 55 bits, see alignment 4.4e-19 amino acids 585 to 634 (50 residues), 25 bits, see alignment 9.9e-10 amino acids 645 to 689 (45 residues), 21.4 bits, see alignment 1.4e-08 amino acids 693 to 741 (49 residues), 33.6 bits, see alignment 2e-12 amino acids 792 to 824 (33 residues), 15.1 bits, see alignment (E = 1.3e-06) amino acids 839 to 884 (46 residues), 34.5 bits, see alignment 1.1e-12

Best Hits

Swiss-Prot: 56% identical to GYRA_GRABC: DNA gyrase subunit A (gyrA) from Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 86% identity to sil:SPO2051)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7EZ60 at UniProt or InterPro

Protein Sequence (908 amino acids)

>PGA1_c15140 DNA gyrase subunit GyrA (Phaeobacter inhibens DSM 17395)
MDENKPERPKYDGPTVSIESEMRTSYLDYAMSVIVSRAIPDLRDGLKPVHRRILYAMHEV
GNSHDKPYRKSARPVGDVMGKYHPHGDSAIYDALVRMAQDFSMSLPLLDGQGNFGSMDGD
NPAAMRYTEVRMDKPAASILADIEKDTVDFQDNYDGKDREPTVLPARFPNMLVNGAGGIA
VGMATNIPPHNLGEVIDATLALIEDPDLTSEQLIDYVPGPDFPTGGVMLGRSGARKAYLE
GRGSVVIRAKTRVEEIRKDRYAIVVEEIPYQVNKAAMIEKIAEQVREKKIEGVSHVQDES
DRNGVRVVVELKRDATAEVVLNQLYRFSPMQTHFGCNMLALNGGRPEQLTLRRFLTSFLD
FREDVIARRTAYDLRKARERSHILCGLAVAVSNVDEVVATIRASADAAEARHKLMTRRWP
AVDIAPYIRLIDDPTHTMNDDGTYNLSEVQARAILELRLQRLTQIGVKEVTDELEELAGK
IKEYLEILSSRERILGIISDELREVRDNFAVPRRTEIVDWSGDMEDEDLIEREDMVVTIT
SGGYIKRTPLVDFRAQKRGGKGLSGMQTKEEDVVTNLFVANTHTQLLFFTTDGMVYKLKT
WRLPQGGRTSKGKAIVNILPIPPGVSIAAIMPVDVPDDEWENLQVVFATSGGDVRRNRLS
DFTNVRRNGKIAMKLPEDGSVKLVNARICSEDDDVMLVTNSGRAIRFRSTDVRVFNSRES
TGVRGIRLVSEGDEVVSMSVIRHFSAEPWERNAFIKRFRSELGGEVAEDENGEESEGSLS
EERYQEMLAANDLLVTINRSGVGNLTSAHDYRVSGRGGQGVGAMKGGAIVATFPVELEDQ
IMLATSKGQSIRCPVNGISFRSRSAGGVRVFNTAKGEEVVSVAWIAERDDEDAEVPTSEG
TSEPAETT