Protein Info for PGA1_c15100 in Phaeobacter inhibens DSM 17395
Annotation: tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K02533, tRNA/rRNA methyltransferase [EC: 2.1.1.-] (inferred from 79% identity to sit:TM1040_1336)Predicted SEED Role
"tRNA:Cm32/Um32 methyltransferase"
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Anthocyanin biosynthesis
- Benzoxazinone biosynthesis
- Biosynthesis of alkaloids derived from shikimate pathway
- Carotenoid biosynthesis - General
- Flavonoid biosynthesis
- Histidine metabolism
- Insect hormone biosynthesis
- Naphthalene and anthracene degradation
- Phenylpropanoid biosynthesis
- Porphyrin and chlorophyll metabolism
- Tryptophan metabolism
- Tyrosine metabolism
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.1.1.-
Use Curated BLAST to search for 2.1.1.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See I7DQ64 at UniProt or InterPro
Protein Sequence (248 amino acids)
>PGA1_c15100 tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ (Phaeobacter inhibens DSM 17395) MPSDTPQPAFVLVRPQMGENVGAAARAMWNFGLDRMRIVAPRDGWPNPKAVAMASGAGRL LDEARLAEDLAGAIEDCDFVFATTARPRELTKPVYSPEAAMKLAAEKIAAGEKVAVMFGP ERAGLENEDIARANAIISVPVNPEFASLNLGQCVLLTAYEWRRQVTEVAPVVTDLGKSDW ASHADVDALVNHYEDRLDDAGFFFPPDKAPSMKTVFRNMFSRMPLTRADVQMLHGIMRQM VRWKEKRD