Protein Info for Psest_1519 in Pseudomonas stutzeri RCH2

Annotation: DNA polymerase elongation subunit (family B)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 791 PF21474: DNApolII_N" amino acids 11 to 46 (36 residues), 76.3 bits, see alignment (E = 2.5e-25) PF22587: DNApolII_insertion" amino acids 47 to 107 (61 residues), 92.4 bits, see alignment 2.7e-30 PF03104: DNA_pol_B_exo1" amino acids 203 to 302 (100 residues), 30.3 bits, see alignment E=5.6e-11 PF00136: DNA_pol_B" amino acids 382 to 758 (377 residues), 131.4 bits, see alignment E=8.8e-42

Best Hits

Swiss-Prot: 68% identical to DPO2_ECOLI: DNA polymerase II (polB) from Escherichia coli (strain K12)

KEGG orthology group: K02336, DNA polymerase II [EC: 2.7.7.7] (inferred from 77% identity to cti:RALTA_A1553)

MetaCyc: 68% identical to DNA polymerase II (Escherichia coli K-12 substr. MG1655)
3.1.11.-; DNA-directed DNA polymerase. [EC: 2.7.7.7]

Predicted SEED Role

"DNA polymerase II (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJB0 at UniProt or InterPro

Protein Sequence (791 amino acids)

>Psest_1519 DNA polymerase elongation subunit (family B) (Pseudomonas stutzeri RCH2)
MGRVMSQQQRGFILTRHWRDTPAGTEISFWLATDDGPRQVCVPHQHSVAFVPEEQHALAE
PLLRRERDAELRPLALCDFRHRPVMGLYCRSYRRLLDCARLLRDAGVDVYEADIRPPERY
LMERFITAPVSFTDAPAGRNLVNTQLKPASDYRPQLRLVSLDIETTARGDLYSIALEGCG
QRQVYMLGPENAQGTAVDFALEYCESRRELLERLNAWFAAHDPDAIIGWNLVQFDLRVLH
EHSQRLQVPLRLGRGGEEMEWREHGGRGNHFFAATAGRLIIDGIEALRSATWSFPSFSLE
NVAQTLLGEGKAIDNPYQRMAEIDRMFAQDKPALARYNLKDCELVTRIFAHTELLTFLLE
RATVTGLAADRSGGSVAAFEHLYMPLMHRQGFVAPNLGERQAEASPGGFVMNSQPGLYES
VLVLDYKSLYPSIIRTFLIDPVGLIEGLRHPNDESSIPGFRGARFSRTRHCLPAIVERVW
EGRETAKREHNKPLSQALKIIMNAFYGVLGSSGCRFFDTRLASSITLRGHEIMRRTRELI
EAQGHAVIYGDTDSTFVWLRKAHSDGEAACIGRELVQHINDWWCEHLQVEYGLASALELQ
YETHFRRFLMPTIRGAEEGSKKRYAGLVTRPDGSDGMVFKGLETVRTDWSPLAQRFQQEL
YQRIFHRQPHRDYVREYVRRTLAGELDDLLIYRKRLRRRLDDYQRNVPPHVRAARLADEF
NERQGRPRQYQNGGWISYLITVAGPEPLETRSSVIDYDHYVTRQLQPVADAILPFVGDDF
ASLVDGQLGLF