Protein Info for Psest_1502 in Pseudomonas stutzeri RCH2

Annotation: tol-pal system beta propeller repeat protein TolB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR02800: Tol-Pal system beta propeller repeat protein TolB" amino acids 19 to 429 (411 residues), 515.9 bits, see alignment E=4.1e-159 PF04052: TolB_N" amino acids 25 to 127 (103 residues), 105 bits, see alignment E=4.5e-34 PF07676: PD40" amino acids 198 to 228 (31 residues), 30 bits, see alignment (E = 7.7e-11) amino acids 237 to 272 (36 residues), 45.7 bits, see alignment 9.1e-16 amino acids 280 to 315 (36 residues), 48.9 bits, see alignment 8.9e-17 amino acids 372 to 400 (29 residues), 16.1 bits, see alignment (E = 1.8e-06)

Best Hits

Swiss-Prot: 81% identical to TOLB_PSEF5: Tol-Pal system protein TolB (tolB) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K03641, TolB protein (inferred from 97% identity to psa:PST_2803)

Predicted SEED Role

"tolB protein precursor, periplasmic protein involved in the tonb-independent uptake of group A colicins" in subsystem Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJ99 at UniProt or InterPro

Protein Sequence (433 amino acids)

>Psest_1502 tol-pal system beta propeller repeat protein TolB (Pseudomonas stutzeri RCH2)
MINSIRGLLVLLCCLAGIAVAQEKNIVVTSGADRAIPIAVVPFGWQGGTVLPEDMAEIVG
NDLRNSGIFQPIPRQNMISMPTRGSEIIYRDWKALGAQYVMVGSIEPAGGRLQVRYEVFN
VTTEQQVMTGTVGGSPDQLRDMAHHAADQSFEKLTGIKGAFSTRLLYVTVERLGGANTRY
TLQRSDYDGARAVTLLQSREPILSPRYSPDGRRIAYVSFEQKRPRIFVQHIDTGRREQIT
NFEGLNGAPAFSPDGNRLAFVLSKDGNPEIYVMDLGSRQLQRLTNHYAIDTEPFWGADGQ
TIYFTSDRAGKPQVYKQRLGSNSAERVTFVGNYNANPKLSADEKTLVMIHRQDGYTNFKV
AAQDLQRGNLRILSDTSLDESPTVAPNGTMLIYATRQQGRGVLMLVSINGRVRLPLPTAQ
GEVREPSWSPYLN