Protein Info for Psest_0146 in Pseudomonas stutzeri RCH2

Annotation: Uncharacterized protein involved in outer membrane biogenesis

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 741 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF05170: AsmA" amino acids 1 to 627 (627 residues), 451.5 bits, see alignment E=8.9e-139

Best Hits

KEGG orthology group: K07289, AsmA protein (inferred from 90% identity to psa:PST_4096)

Predicted SEED Role

"A/G-specific adenine glycosylase (EC 3.2.2.-)" in subsystem DNA repair, bacterial (EC 3.2.2.-)

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.-

Use Curated BLAST to search for 3.2.2.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GDE1 at UniProt or InterPro

Protein Sequence (741 amino acids)

>Psest_0146 Uncharacterized protein involved in outer membrane biogenesis (Pseudomonas stutzeri RCH2)
MKSLGKILGLVFLGLLLILVAAGFALTHLFDPNDYKDEIRDLARSKAGLELDIKGDIGWS
LFPWLGLELHETTLASAQTPEQPFADLRMLGLSVRVMPLLSREVQMSDIKVNGLNLTLNR
DEKGRGNWEGVGQPAKQPEQPAQTPKASEPSPEQADQAPASRNKPLTLDIDSLTVSDARV
TYHDAKTGQQYSAESIELTTGAIREGSSIPVKLNAFFGSNQPVMRARTELQGALRFDNQL
KRYQFEDMRLSGEASGEPLQGQTLTFSAQGQLLVDLAAQIAEWNSLKFTANQLRGLGELK
ARDLDKEAKISGALTVAQFNLREFLQGTGQKLPAMADGATLSKAELVSRLSGTPNSIALE
ELTLKLDDSTFTGRLAVSDFARQALRVDLKGDRFNLDRYLPPPSKEQAAEAARKSEVKST
QASAIGSGTTPLPNAPTQQAWSDATVLPLEQLRKLDTEVNLAIGSLTAMKIPLDGFNLKA
RTRNGLLTLQEMRAGLYGGRLDGSASLDVRPALPLLTVQNRFNGVPVERLLESQGEDVVV
KGLLNLDADLRTQGNSEKAWIDNLNGKVGFIIDNGVLVDANLEQQLCRAIATLNRKPLAS
DPRSKDTPFRELKGNLTLRNGVASNPDLKASIPGLTVNGKGDVDLRVLGMDYRVGILIEG
DKGAMPDPACQVNERYVGIEWPLRCRGPLELGAKACRFDNDALGKVAARLAGEKLNEKIE
EKLGDKVSPELKDALKGLFKR