Protein Info for Psest_0145 in Pseudomonas stutzeri RCH2

Annotation: A/G-specific adenine glycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 355 TIGR01084: A/G-specific adenine glycosylase" amino acids 5 to 273 (269 residues), 358.8 bits, see alignment E=1.1e-111 PF00730: HhH-GPD" amino acids 35 to 169 (135 residues), 79 bits, see alignment E=5e-26 PF00633: HHH" amino acids 100 to 128 (29 residues), 33.6 bits, see alignment (E = 3.4e-12) PF14815: NUDIX_4" amino acids 237 to 343 (107 residues), 75.5 bits, see alignment E=4.4e-25

Best Hits

Swiss-Prot: 52% identical to MUTY_ECOLI: Adenine DNA glycosylase (mutY) from Escherichia coli (strain K12)

KEGG orthology group: K03575, A/G-specific adenine glycosylase [EC: 3.2.2.-] (inferred from 96% identity to psa:PST_4097)

MetaCyc: 52% identical to adenine DNA glycosylase (Escherichia coli K-12 substr. MG1655)
RXN0-2661 [EC: 3.2.2.31]

Predicted SEED Role

"A/G-specific adenine glycosylase (EC 3.2.2.-)" in subsystem DNA repair, bacterial (EC 3.2.2.-)

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.-

Use Curated BLAST to search for 3.2.2.- or 3.2.2.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GG65 at UniProt or InterPro

Protein Sequence (355 amino acids)

>Psest_0145 A/G-specific adenine glycosylase (Pseudomonas stutzeri RCH2)
MSPEQFGTAVLDWFDSHGRKDLPWQQGITPYRVWVSEIMLQQTQVSTVLGYFDRFMDALP
SVEALAAAEEDEVLHLWTGLGYYSRARNLHKTAKLIVVEHGGVFPADVDKLAELPGIGRS
TAGAIASISLGLRAPILDGNVKRVLARYVAQQGYPGEPKVARQLWEVAERFTPQQRVNHY
TQAMMDLGATLCTRSRPSCLLCPLKDGCRAHLLGRETDFPVPKPRKALPQKRTLMPLLAN
RDGAILLYRRPSTGLWGGLWSLPELDDLAALDPLAERHALQLEERRELPGLTHTFSHFQL
AIEPWLIRVKSAPDAVAEADWLWYNLATPPRLGLAAPVKTLLKRAAAELHAGENP