Protein Info for Psest_1481 in Pseudomonas stutzeri RCH2

Annotation: Predicted membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 410 transmembrane" amino acids 29 to 55 (27 residues), see Phobius details amino acids 66 to 83 (18 residues), see Phobius details amino acids 93 to 112 (20 residues), see Phobius details amino acids 133 to 157 (25 residues), see Phobius details amino acids 168 to 193 (26 residues), see Phobius details amino acids 204 to 228 (25 residues), see Phobius details amino acids 234 to 265 (32 residues), see Phobius details TIGR00765: YihY family inner membrane protein" amino acids 11 to 266 (256 residues), 244.6 bits, see alignment E=7.8e-77 PF03631: Virul_fac_BrkB" amino acids 22 to 264 (243 residues), 209.7 bits, see alignment E=3.1e-66

Best Hits

Swiss-Prot: 96% identical to Y2825_PSEU5: UPF0761 membrane protein PST_2825 (PST_2825) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K07058, membrane protein (inferred from 96% identity to psa:PST_2825)

Predicted SEED Role

"Ribonuclease BN (EC 3.1.-.-)" in subsystem LMPTP YfkJ cluster or tRNA processing (EC 3.1.-.-)

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-

Use Curated BLAST to search for 3.1.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GKX9 at UniProt or InterPro

Protein Sequence (410 amino acids)

>Psest_1481 Predicted membrane protein (Pseudomonas stutzeri RCH2)
MHQRIDNMLEFGRFLVRRFLADQGPHSAAALTYTTLFAVVPMMTVTFAMLSAIPAFKGVG
EQIQFYIFNNFIPSTGATIQEYLLAFTSQARQLTWFGVGFLMATALMMLLTIEKAFNTIW
RVRQPRRGVSSFLLYWAILSLGPLLLGAGFATSTYIASLSLISGPYALIGVGTLIKVMPL
LLSVAAFTLIYAAVPNTRVPLRHAIVGGVFTAVLFEAAKQLFGVYVSYFPSYQLIYGAFA
AVPLFLLWVYLSWMIVLFGAELVCGLSSSQQWRRRPLPRLLVMLMLLRNLHQRQQDGREM
HLRDLHKAGLRLPEDEWDEILGFFEQEQLVCRTGSGGWVLCRDLSHYSLDQLLRCNPWPL
SARVELPGQLDEPWYPTLRRSLELLQQEQGNLFGGSLADWLQAGGDKKRQ