Protein Info for HP15_1333 in Marinobacter adhaerens HP15

Annotation: error-prone DNA polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1039 TIGR00594: DNA polymerase III, alpha subunit" amino acids 7 to 984 (978 residues), 552.9 bits, see alignment E=8.4e-170 PF02811: PHP" amino acids 13 to 111 (99 residues), 42.8 bits, see alignment E=1.8e-14 PF07733: DNA_pol3_alpha" amino acids 284 to 538 (255 residues), 296.4 bits, see alignment E=5.5e-92 PF17657: DNA_pol3_finger" amino acids 541 to 707 (167 residues), 194.6 bits, see alignment E=2e-61 PF14579: HHH_6" amino acids 781 to 867 (87 residues), 62 bits, see alignment E=1.3e-20 PF01336: tRNA_anti-codon" amino acids 949 to 1018 (70 residues), 40.1 bits, see alignment 7.1e-14

Best Hits

Swiss-Prot: 56% identical to DNAE2_VIBVY: Error-prone DNA polymerase (dnaE2) from Vibrio vulnificus (strain YJ016)

KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 84% identity to maq:Maqu_0973)

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PIM2 at UniProt or InterPro

Protein Sequence (1039 amino acids)

>HP15_1333 error-prone DNA polymerase (Marinobacter adhaerens HP15)
MSVQQYAELFCFSNFTFLTGASHPHELAERAHELGYTALAITDACSVAGIPRAWAALAES
PVKLITGSWFELSDAPAGATQPRFILLARTRKGYGQLCQLITTGRRRAEKGHYQLFYRDI
ETHTLNDCLCLWLPPSPTEADSDQALACGEWLARLFDPRIWIAAARTLESGEEQRLARIH
WLADQLRIPVAAVGEVHMHSRERQPLQDVLTALRNHTNLENAGHCLFQNGERYLRPLPVL
QRLFPEAWLHETLAIASQCTFEPGSLRYEYPPDLVPEGETPAGYLKRLTREGERRRYPDG
TPLQVQSLIRKELGLISEMNYEHYFLTIHDIVDFARSRGILCQGRGSAANSAVCYCLGIT
EVNPARVELLFERFISKDRNEPPDIDVDFEHERREEVIQYIYRRYTRERAALAATVIRYR
PKSAIRDVGKALGFDPALVEQLLEGIDWRDKATNWRQQILDKKITRNPQVADQFFTLVNT
LLGFPRHLSQHVGGFVISAGPLAELVPVENAAMTDRTVIQWDKDDLESLGLMKVDVLALG
MLSAIRKALELISDEKGQPFRIQDIPQEDRDTYAMLQTGDSIGVFQVESRAQINMLPRLK
PETYYDLVIEVAIVRPGPIQGDMVHPYLRRKHGLEPVDYPNDAVRKVLERTLGVPIFQEQ
VIKLAMVAAGFSAGEADQLRRAMAAWKSHGDLTPFREKLVTGMLERGHDADFAERLYQQI
CGFGGYGFPESHAASFALLVYVSAWIKRHYPAAFYCALLNSQPMGFYSPSQLVQDARRHN
VTVLPPDVNASQWDHTLQGENRHLRLGLRIIQGLSVYGAERIHQNRPAEGYRSASELRRL
AALNQRDMELLAGANAMPGFTANRHQAYWQLLDHEQPTELFAEETAVDYQPDYCEQLPEP
SEGQNVLADYASQGLTLQRHPLALLRDQGHLKFCLSAEQLKSTKAGIPVQVAGLVTGRQR
PGSASGVTFVTLEDETGNVNVVVWLETARRQRKPLLTARLLHVKGVLERQGDIVHVMAGR
LSDLSHLIQSLPVNSRNFH