Protein Info for Psest_1383 in Pseudomonas stutzeri RCH2

Annotation: DNA topoisomerase III, bacteria and conjugative plasmid

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 646 TIGR01056: DNA topoisomerase III" amino acids 1 to 643 (643 residues), 703.3 bits, see alignment E=2e-215 PF01751: Toprim" amino acids 2 to 128 (127 residues), 57.6 bits, see alignment E=1.2e-19 PF01131: Topoisom_bac" amino acids 149 to 590 (442 residues), 389 bits, see alignment E=3.1e-120

Best Hits

KEGG orthology group: K03169, DNA topoisomerase III [EC: 5.99.1.2] (inferred from 95% identity to psa:PST_2913)

Predicted SEED Role

"DNA topoisomerase III (EC 5.99.1.2)" in subsystem DNA topoisomerases, Type I, ATP-independent (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GKL7 at UniProt or InterPro

Protein Sequence (646 amino acids)

>Psest_1383 DNA topoisomerase III, bacteria and conjugative plasmid (Pseudomonas stutzeri RCH2)
MQLYLCEKPSQARDIAKVLGANRRGDGCLQGAGVTVTWCIGHLLETAPPDAYDPRYKRWV
LADLPIVPERWKMLVKPKTASQFKAVKRLLGECSELVIATDADREGEMIARELVEHCRYR
GPIRRLWLSALDDASIRKALAALKPGAETFNLYHAALGRSRADWLIGMNMSRLFTLLGRQ
SGYQGVLPVGRVQTPTLRLVVDRDRSIADFIPVPFWAIDVQLLADGTAFTAQWQAPDDYC
DDQGRCLDQARAQQAAEAMRNAACARLLKLKTERQREAAPLPFDLGTLQEVCSKKLGLGA
QETLDIAQALYETHKLITYPRSDCGYLPLSQHGEARAIVAALAQSDPTLAALEPHLDLSR
RSRAWNDAKVGAHHGIIPTAAVRGLERLSGRPRAVYELIRARYLAQFLPNHEYDRTQADF
DCAGQALRAVGKRIVEPGWKRAMPEALAPARGNREAPAPQSLPTLQQGQDYAVGEINLKD
QQTHPPKPFTEGDLIKAMKNVAKLVDDPRLKQKLKDTTGIGTEATRAGIIQGLLDRGYLL
RQGKALAATPAAFSLIDAVPRPIADPGTTAIWEQALDMVQSGEMPLEEFVAKQSAWMSKL
VERCAGLRMTISGPPVAAGRGGKPWKKKRSAAPRKPARRRKPAAAD