Protein Info for HP15_1296 in Marinobacter adhaerens HP15

Annotation: valine-pyruvate transaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 425 PF00155: Aminotran_1_2" amino acids 69 to 407 (339 residues), 91.3 bits, see alignment E=3.7e-30

Best Hits

Swiss-Prot: 51% identical to AVTA_ECOLI: Valine--pyruvate aminotransferase (avtA) from Escherichia coli (strain K12)

KEGG orthology group: K00835, valine--pyruvate aminotransferase [EC: 2.6.1.66] (inferred from 93% identity to maq:Maqu_0943)

MetaCyc: 51% identical to valine--pyruvate aminotransferase (Escherichia coli K-12 substr. MG1655)
Valine--pyruvate transaminase. [EC: 2.6.1.66]; 2.6.1.- [EC: 2.6.1.66]

Predicted SEED Role

"Valine--pyruvate aminotransferase (EC 2.6.1.66)" in subsystem Alanine biosynthesis or Branched-Chain Amino Acid Biosynthesis or Pyruvate Alanine Serine Interconversions (EC 2.6.1.66)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.66

Use Curated BLAST to search for 2.6.1.66

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PII5 at UniProt or InterPro

Protein Sequence (425 amino acids)

>HP15_1296 valine-pyruvate transaminase (Marinobacter adhaerens HP15)
MKLSAFGRKFTADAGITSLMDDLGNAMASGDDMIMMGGGNPGHIPEIQQRVQEILADMSR
NEGDVRRLVGIYDPPQGEKQFIASLAELLNREYGWGLKPENIALTNGSQAAFFMLFNMFG
GDYGDGNRKHILLPLAPEYIGYADAGIEPGLFHAVQPDISFTDAHEFKYRVDFDAVEVTG
ETGAICVSRPTNPTGNVVTDEELARLEELARQHDIPLIVDGAYGTPFPSLLFVDAAPTWN
DQIILCLSLSKLGLPAARTGIVIAAEPVIKALSGINAIMNLATGSFGAMLAEPLVRSGEI
LSLSRDVVCPFYKAKMERAVSAFREAMGEDSCRWYVHKPEGAMFLWLWFPDLPISSLELY
QRLKSRGVLVVSGHYFFPGLPEDGWRHRHECLRVTYSQDDDRVAEGLRIIADEVKAVWRE
AGREV