Protein Info for HP15_1292 in Marinobacter adhaerens HP15

Annotation: methionyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 676 PF00133: tRNA-synt_1" amino acids 4 to 246 (243 residues), 46 bits, see alignment E=8.4e-16 TIGR00398: methionine--tRNA ligase" amino acids 11 to 535 (525 residues), 601.7 bits, see alignment E=1.3e-184 PF09334: tRNA-synt_1g" amino acids 11 to 398 (388 residues), 529.1 bits, see alignment E=1.6e-162 PF19303: Anticodon_3" amino acids 415 to 515 (101 residues), 26.7 bits, see alignment E=1.1e-09 TIGR00399: methionine--tRNA ligase, beta subunit" amino acids 540 to 675 (136 residues), 151.4 bits, see alignment E=1.4e-48 PF01588: tRNA_bind" amino acids 581 to 673 (93 residues), 89.7 bits, see alignment E=2.7e-29

Best Hits

Swiss-Prot: 90% identical to SYM_MARHV: Methionine--tRNA ligase (metG) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 90% identity to maq:Maqu_0939)

MetaCyc: 63% identical to methionine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Methionine--tRNA ligase. [EC: 6.1.1.10]; 6.1.1.10 [EC: 6.1.1.10]; RXN-23924 [EC: 6.1.1.10]

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PII1 at UniProt or InterPro

Protein Sequence (676 amino acids)

>HP15_1292 methionyl-tRNA synthetase (Marinobacter adhaerens HP15)
MTQASKQQRDILVTSALPYANGPIHLGHLLEYIQTDIWVRYQKMRGQNCYYVCADDAHGT
AIMLRAEREGITSEQLIDRIREEHQEDFAGFHILFDNYYTTHSEENRYFSEYIYRQLQEN
GHIATRKITQSYDPEKNMFLADRFIKGTCPKCKTEDQYGDNCEACGATYTPAELINPRSA
VSGATPIEKESEHYFFKLPDFQDFLAKWTRSGTLQPQVANKLAEWLDAGLQEWDISRDAP
YFGFEIPDAPGKYFYVWLDAPIGYLASFKNLCNREGIDFEHFWKADSTAEVYHFIGKDII
NFHALFWPSMLHDAGFRTPTAVWAHGFVTVNGKKMSKSRGTFIMARTYLDHLNPEYLRYY
FAAKLTGSVDDMDLNLEDFAARVNSDLVGKVVNIASRSAGFITKRFDGQLGQVTEHAKLK
EFIEAGKEIEEYYESREFGRAMRRIMELADIANQYVNDEQPWVIAKQEGQDENLQAICTN
AINMFHLLMTYLAPVLPETAKASEAFLNAPLDWNNRSERLENHGINKFKPLMNRVDMAQI
EKMLDSSKEELPAATGKPAPAANLEPIADEIEFGDFAKVDLRVVKIVKAEHVEGADKLLR
LTLDIGHGERNVFAGIKSAYKPEDLEGRLTVMVANLKPRKMKFGMSEGMVLAAGPGGKDI
FILSPDSGATPGMRIM