Protein Info for Psest_1350 in Pseudomonas stutzeri RCH2

Annotation: Metal-dependent hydrolases of the beta-lactamase superfamily III

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 330 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details PF00753: Lactamase_B" amino acids 24 to 82 (59 residues), 37.5 bits, see alignment E=2.5e-13 PF12706: Lactamase_B_2" amino acids 32 to 149 (118 residues), 39.7 bits, see alignment E=4.4e-14

Best Hits

Swiss-Prot: 60% identical to RNZ_PSEE4: Ribonuclease Z (rnz) from Pseudomonas entomophila (strain L48)

KEGG orthology group: K00784, ribonuclease Z [EC: 3.1.26.11] (inferred from 89% identity to psa:PST_2944)

Predicted SEED Role

"Ribonuclease Z (EC 3.1.26.11)" in subsystem tRNA processing (EC 3.1.26.11)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.26.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJD4 at UniProt or InterPro

Protein Sequence (330 amino acids)

>Psest_1350 Metal-dependent hydrolases of the beta-lactamase superfamily III (Pseudomonas stutzeri RCH2)
MDLLFLGTSSGTPTRARNVTALALLEDTGKSWYLIDCGEGTQHRLLRTPLSLHDLRAICI
THVHGDHCYGLPGLLASAGMMGRKATLTIIAPQGIETWVRTTLDMSQSWLGYELDFRAVE
SLDEWRSPNMRIEATALSHRVPCYGYSFTEARPDPRLDIERLERDGVPRGPLWGQLARGF
DIEHEGRVLRSDDYLSFSRAPQRIVIGGDNDRPDLLADACRGAQLLVHEATYTEAVANDA
RNDFGHSTAASVARFAQAVGLPNLVLTHFSARYQKHPGRGHSIEDLRAEANVLYSGQLLL
AEDFMRLHLGKDGQLITVEASDPRSSRSPR