Protein Info for Psest_1344 in Pseudomonas stutzeri RCH2

Annotation: rRNA methylases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 243 PF22435: MRM3-like_sub_bind" amino acids 5 to 84 (80 residues), 50.3 bits, see alignment E=2.6e-17 PF00588: SpoU_methylase" amino acids 104 to 239 (136 residues), 92.1 bits, see alignment E=3.6e-30

Best Hits

KEGG orthology group: K03437, RNA methyltransferase, TrmH family (inferred from 90% identity to psa:PST_2950)

Predicted SEED Role

"FIG011178: rRNA methylase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJC8 at UniProt or InterPro

Protein Sequence (243 amino acids)

>Psest_1344 rRNA methylases (Pseudomonas stutzeri RCH2)
MKFDDIKKLHQKKYRAEFGHFLVEGEHLALELQKAALHNPQLARSELYVTNAYEHWQSPF
KTHLISDRQMAQIADTKTPQGIVALVPMPATGASLAAPVAGERAIYLHEIQDPGNLGTIL
RTLAWFGNFRCLLSPGSVDPYNPKVVRSSMGAIFHAPMELDVELDSLRTRFERIACLDMH
GEPVRSAGFRAFDCYLFGNEARGVPRDQLNALDAQPFTIPGCGAIESLNLAATVNMCAYE
LSR