Protein Info for Psest_1339 in Pseudomonas stutzeri RCH2

Annotation: ATP-dependent helicase HrpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1360 TIGR01967: RNA helicase HrpA" amino acids 12 to 615 (604 residues), 1095.2 bits, see alignment E=0 amino acids 671 to 1357 (687 residues), 903.3 bits, see alignment E=1.4e-275 PF00270: DEAD" amino acids 81 to 228 (148 residues), 27.1 bits, see alignment E=1e-09 PF00271: Helicase_C" amino acids 294 to 410 (117 residues), 43.7 bits, see alignment E=8.7e-15 PF04408: HA2_N" amino acids 475 to 500 (26 residues), 25 bits, see alignment (E = 4.9e-09) PF21010: HA2_C" amino acids 501 to 560 (60 residues), 39 bits, see alignment (E = 2.9e-13) PF07717: OB_NTP_bind" amino acids 681 to 756 (76 residues), 69.6 bits, see alignment 6.2e-23 PF11898: DUF3418" amino acids 770 to 1357 (588 residues), 684.2 bits, see alignment E=5.2e-209

Best Hits

KEGG orthology group: K03578, ATP-dependent helicase HrpA [EC: 3.6.4.13] (inferred from 85% identity to avn:Avin_16270)

Predicted SEED Role

"ATP-dependent RNA helicase HrpA (EC 3.6.4.13)" (EC 3.6.4.13)

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJC3 at UniProt or InterPro

Protein Sequence (1360 amino acids)

>Psest_1339 ATP-dependent helicase HrpA (Pseudomonas stutzeri RCH2)
MTDATPAIDTLLKNLDQALFADRHRLRRQLHELRKQPDEAKLAQWLERFQASVAKVEARR
RSVPSIRYDDSLPIAAKRDEIKAALEKHQVLVIAGETGSGKTTQLPKICLEIGRGVHGLI
GHTQPRRLAARSVATRVAEEIGTPLGELVGYQVRFEDQSKDSSLIKLMTDGILLAETQHD
RFLERYDTIIVDEAHERSLNIDFLLGFLKTLLPRRPDLKVIITSATIDLQRFSEHFDGAP
IVEVSGRTYPVETWYRPLAAEIDEDGNRVEDDLTVDQGILAALDEITAHEQSVGKRPGDV
LVFLPGEREIRDAAEVLRKANLKFTEVLPLYARLTPAEQQKIFQPRPGRKIVLATNVAET
SLTVPGIRYVIDSGTARISRYSYRAKVQRLPIEAVSQASANQRKGRCGRVEPGICIRLYS
EEDFLGRPEFTDPEILRTNLAAVILQMLHLRLGDIQDFPFIEPPDGKAISDGFNLLQELS
AVNRENQLTPMGRQLARLPIDPRLGRMLLEAAQQGSLAEVLIVASALSVQDVRERPADRQ
QAADQAHAQWKDPDSDFAALINLWRGFEEKRQELGSNPLRTWCRKNFLNYLRLREWRDAH
RQLTLIARELKLGSGRSADGSGQAAHADKARRVEDGEASSALSAGVSTQPAPTKDTKVNV
ILRQQAEASEAAQKAKSYASVHKAILAGLLSQIGNKTEEGDFLGARQRRFWVHPSSVIGR
KKPNWLMAAELVETTKLFARMVAKIEPDWIEPLAGHLIKKNHFEPHWEKKRGQVVAYEQV
TLYGMIVVGRRPVHYGPIDPPAARELFIREGLVRGEINSRARALSANRELLERLDELEAK
ARRRDILADEETLFDYYDARLPADIYQTASFENWYKRESAKNPQLLIMREEDVLAREASE
VTAAQYPDYLRIGELQLPLEYHFEPNHPRDGVTLRVPAPLLPQLRSERLDWLVPGLLETK
AVALVRNLPKALRKNFVPVPDFVGAALARIAFGEGSLPEALGRELLRMTGARVSDDAWAE
AAAGLESHLKMNIEVVDARGKFLGEGRDLAELTARFAEASQAALAPPQQKAEQKPVEAKG
FAQVAEKAQAKMAGLSMTVYPALVEEAGVVKEGRFPTQAEAEWQHRRALQRLLLQQLAEP
AKYLRNKLPGLTELGLLYRDMGKVDALVEDILLASLDSCILDGEAQLPRDGAALASLAER
KRGDWTAHAERLARLTLEILKHWHGLQKRFKGKIDLAQAVALNDIKAQLGNLVYPGFVRE
TPAEWLKEYPRYLKAIEQRFEKIGAQLQRDRVWSGELAGYWEQYQTRLNKHLQEGKRDAE
LALYRWMLEEYRVSLWAQQLGTRMAVSDKRLNKQWSQVEP