Protein Info for Psest_1332 in Pseudomonas stutzeri RCH2

Annotation: alpha-1,6-glucosidases, pullulanase-type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1039 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details transmembrane" amino acids 828 to 847 (20 residues), see Phobius details PF03714: PUD" amino acids 39 to 136 (98 residues), 66.6 bits, see alignment E=8e-22 TIGR02103: alpha-1,6-glucosidases, pullulanase-type" amino acids 148 to 1036 (889 residues), 1179.5 bits, see alignment E=0 PF17967: Pullulanase_N2" amino acids 151 to 263 (113 residues), 86.9 bits, see alignment E=4.6e-28 PF02922: CBM_48" amino acids 271 to 356 (86 residues), 43.4 bits, see alignment E=8.4e-15 PF18494: Pullulanase_Ins" amino acids 453 to 520 (68 residues), 68.8 bits, see alignment 1.2e-22 PF11852: Pullul_strch_C" amino acids 874 to 1034 (161 residues), 178.1 bits, see alignment E=2.6e-56

Best Hits

KEGG orthology group: None (inferred from 90% identity to psa:PST_2963)

Predicted SEED Role

"putative pullulanase precursor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GKG1 at UniProt or InterPro

Protein Sequence (1039 amino acids)

>Psest_1332 alpha-1,6-glucosidases, pullulanase-type (Pseudomonas stutzeri RCH2)
MLIRSIAYRRLAALVLGGLSLAMQPASAAPLDHGSDEALLYYQRADGDYNGWGPHLWNTP
DCNGSATETSWAQPLTAAGTDPEYGAFYRIPLSVDASCLNLIMHKGDEKDLGGGDLTWRF
DELGRRVFTVSGNPQLSSTPLSGSAVAIKGARAHWLDPFTLALVNGAPGASRLELRYASD
ASIRIDGEARTVSGGQSLVLQPSALRNGLKRQHPHLANAPAYRITAGARDIRRAVMSQLV
VVAYDADDRVIDATEVQTAGILDMLFAYNGELGATLAAKGVSFKLWAPTAQRVRLHVFDA
AKRLLPGYPKLMQERLGVWSLEGPRSLDRQYYQYEVTAYRPSTGTIETTLVSDPYALSLS
RNSQYAQVVDLNADDLKPAGWDAVRPPRPQRPEANVIYETHLRDFSASDTSLPAALRGTY
AAFTHQHSDSMRHLRDLQKAGLTHVQLLPVFDIATIDEDPARRINLDDPFAKLCDLSDAA
RERWAQYCGAASIRQVLAGFDPASGQAQSLYNDLRGLDDFNWGYDPFHFTAPEGSYASDA
EGVQRIIEFRQMVQALAGNDLATVMDVVYNHTNASGLAEKSVLDKVVPGYYHRRNPTTGA
VETSTCCENTASEHRMMAKLMTDSLKVWARDYKIAGFRFDLMGHHMREQIIDAYRAVRRI
DRDTYFYGEGWEFGEVAGNARGINANQANMAGTGVGTFNDRQRDAVRGGSPFDSGDSIRR
NQGFANGLYLRPNELSAAGAQEKAQLLHAADLIRVGIAGGLRDFQFVTADGSMRKGSEID
YNGQPAGYTLDPQETVNYVSKHDNQTLWDNNQYKFASSLSVADRVRLHLVALSVPLFSQG
VPFIHLGSDILRSKSMQRDSYDSGDWFNAVDFSYQDNNWNKGLPRADKDGDNWPLIRRII
VDPQAKPGPTEIVTAKRRFLELLKIRSDSQLFQLDSAHEVQRRLQFHNTGPEQQPGVIAF
SLADAPHDGRDLDRRYSSLKVVINASAQRARLPGADGYQLHPALKSSIDPISRQAKVVDG
EFQVPAFTTSVFVQPQTRR