Protein Info for HP15_1245 in Marinobacter adhaerens HP15

Annotation: phosphoribosylglycinamide formyltransferase 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01142: phosphoribosylglycinamide formyltransferase 2
  • amino acids 17 to 394 (378 residues), 620 bits, E=6.9e-191
• PF01370: Epimerase
  • amino acids 22 to 84 (63 residues), 22.1 bits, E=2.7e-08
• PF02786: CPSase_L_D2
  • amino acids 124 to 222 (99 residues), 28.2 bits, E=4.1e-10
• PF02222: ATP-grasp
  • amino acids 126 to 297 (172 residues), 176.5 bits, E=1.1e-55
• PF07478: Dala_Dala_lig_C
  • amino acids 129 to 287 (159 residues), 29.6 bits, E=1.5e-10
• PF21244: PurT_C
  • amino acids 324 to 392 (69 residues), 103.3 bits, E=1.2e-33

Best Hits

Swiss-Prot: 84% identical to PURT_MARHV: Formate-dependent phosphoribosylglycinamide formyltransferase (purT) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K08289, phosphoribosylglycinamide formyltransferase 2 [EC: 2.1.2.2] (inferred from 83% identity to maq:Maqu_2478)

MetaCyc: 66% identical to phosphoribosylglycinamide formyltransferase 2 (Escherichia coli K-12 substr. MG1655)
GARTRANSFORMYL2-RXN [EC: 6.3.1.21]; Acetate kinase. [EC: 6.3.1.21, 2.7.2.1, 2.7.2.15]

Predicted SEED Role

"Phosphoribosylglycinamide formyltransferase 2 (EC 2.1.2.-)" in subsystem De Novo Purine Biosynthesis (EC 2.1.2.-)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• Bifidobacterium shunt (15/15 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (6/6 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis II (5/5 steps found)
• ethanolamine utilization (5/5 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (15/19 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (4/4 steps found)
• pyruvate fermentation to acetate and lactate II (4/4 steps found)
• pyruvate fermentation to acetate I (3/3 steps found)
• pyruvate fermentation to acetate II (3/3 steps found)
• pyruvate fermentation to acetate VII (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• L-threonine degradation I (5/6 steps found)
• acetate and ATP formation from acetyl-CoA I (2/2 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• acetylene degradation (anaerobic) (4/5 steps found)
• acetyl-CoA fermentation to butanoate (5/7 steps found)
• pyruvate fermentation to acetate IV (2/3 steps found)
• glycine degradation (reductive Stickland reaction) (1/2 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• mixed acid fermentation (11/16 steps found)
• (S)-propane-1,2-diol degradation (3/5 steps found)
• superpathway of L-threonine metabolism (12/18 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (5/9 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (4/8 steps found)
• methanogenesis from acetate (2/6 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (4/9 steps found)
• superpathway of N-acetylneuraminate degradation (13/22 steps found)
• L-lysine fermentation to acetate and butanoate (4/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (8/17 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (5/13 steps found)
• gallate degradation III (anaerobic) (3/11 steps found)
• purine nucleobases degradation I (anaerobic) (5/15 steps found)
• purine nucleobases degradation II (anaerobic) (11/24 steps found)
• superpathway of methanogenesis (2/21 steps found)
• superpathway of L-lysine degradation (12/43 steps found)

KEGG Metabolic Maps

• Nucleotide sugars metabolism
• One carbon pool by folate
• Propanoate metabolism
• Purine metabolism
• Pyruvate metabolism
• Taurine and hypotaurine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.2, 2.7.2.1

Use Curated BLAST to search for 2.1.2.- or 2.1.2.2 or 2.7.2.1 or 2.7.2.15 or 6.3.1.21

Sequence Analysis Tools

See E4PHP4 at UniProt or InterPro

Protein Sequence (396 amino acids)

>HP15_1245 phosphoribosylglycinamide formyltransferase 2 (Marinobacter adhaerens HP15)
MTDSVRIGTPLKGNAFKVLFCGSGELGKEVVIELQRYGVEVIAVDRYADAPAMQVAHRSH
VIDMLDGASLRSVIEQEKPDLIVPEIEAIATPELVKLEEEGYRVIPTARAVNLTMNREGI
RRLAAEELGLPTSPYRFAGTREEYLAAVNEIGLPLVVKPVMSSSGKGQSTVKTEDDIEAA
WEYAQSGGRAGKGRVIVEGFVDFDYEITLLTVKHRDGVSFCEPIGHRQEDGDYRESWQPQ
PMAPLAYERSAEVARAVVENLGGYGIYGVELFVKEDNVWFSEVSPRPHDTGLVTLVSQDL
SEFALHARAILGLPIPAIRQTGPSASAVILPEGDSASVSYSGLESALSEPDTQLRLFGKP
ELKGRRRMGVALAKGATVDEARKKARDSANSVTVEL