Protein Info for PGA1_c12850 in Phaeobacter inhibens DSM 17395

Updated annotation (from data): Dihydropyrimidinase (EC 3.5.2.2)
Rationale: Specifically important for utilizing Uridine. Automated validation from mutant phenotype: the predicted function (3.5.2.2) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: D-hydantoinase/dihydropyrimidinase Dht

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02033: dihydropyrimidinase
  • amino acids 4 to 452 (449 residues), 597.7 bits, E=8e-184
• PF01979: Amidohydro_1
  • amino acids 47 to 418 (372 residues), 80.4 bits, E=1.6e-26

Best Hits

Swiss-Prot: 53% identical to HYDA_PSEAE: D-hydantoinase/dihydropyrimidinase (dht) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01464, dihydropyrimidinase [EC: 3.5.2.2] (inferred from 94% identity to sit:TM1040_1497)

MetaCyc: 47% identical to dihydropyrimidinase (Arabidopsis thaliana col)
Dihydropyrimidinase. [EC: 3.5.2.2]

Predicted SEED Role

"Dihydropyrimidinase (EC 3.5.2.2)" in subsystem Hydantoin metabolism or Pyrimidine utilization (EC 3.5.2.2)

MetaCyc Pathways

• superpathway of pyrimidine ribonucleosides degradation (5/5 steps found)
• thymine degradation (3/3 steps found)
• uracil degradation I (reductive) (3/3 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pantothenate and CoA biosynthesis
• Pyrimidine metabolism
• beta-Alanine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.2.2

Sequence Analysis Tools

See I7EW42 at UniProt or InterPro

Protein Sequence (484 amino acids)

>PGA1_c12850 Dihydropyrimidinase (EC 3.5.2.2) (Phaeobacter inhibens DSM 17395)
MAKVIRNGTVVTADLTYKADVLIENGVITEIGPNLKGDEELDASGCYVMPGGIDPHTHLE
MPFMGTYSSDDFESGTRAGLAGGTTMVVDFALPNPGESLLDALKRWDNKSTRANCDYSFH
MAVTWWGEQVFDDMKTVIETRGINTFKHFMAYKGALMVNDDELYASFQRLSELGGIAMVH
AENGDVVAELSAKLLAEGNNGPEAHAYSRPPQVEGEATNRAIMIADMAGVPLYVVHTSCE
DSHEAIRRARMQGKRVWGEPLIQHLTLDESEYFNSDWDHAARRVMSPPFRNKQHQDSLWA
GLQSGSLSVVATDHCAFSTEQKRYGVGDFTKIPNGTGGLEDRMPMLWTHGVETGRLTPNE
FVAVTSTNIAKILNCYPKKGAVLVGADADLVVWDPAKTKTISAASQQSAIDYNVFEGKEI
KGLPRYTLTRGHVAVHDGEIRCQEGHGKFLEREANTTVNKALSTWKDLTAPRPVARSGIP
ATGV