Protein Info for PGA1_c12840 in Phaeobacter inhibens DSM 17395

Updated annotation (from data): Beta-ureidopropionase (EC 3.5.1.6)
Rationale: Specifically important for utilizing Uridine. Automated validation from mutant phenotype: the predicted function (3.5.1.6) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: N-carbamoyl-L-amino acid hydrolase AmaB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 TIGR01879: amidase, hydantoinase/carbamoylase family" amino acids 13 to 410 (398 residues), 394.6 bits, see alignment E=2.5e-122 PF04389: Peptidase_M28" amino acids 67 to 149 (83 residues), 22.1 bits, see alignment E=1.7e-08 PF01546: Peptidase_M20" amino acids 84 to 409 (326 residues), 104.9 bits, see alignment E=8.4e-34 PF07687: M20_dimer" amino acids 215 to 314 (100 residues), 29.2 bits, see alignment E=1.1e-10

Best Hits

Swiss-Prot: 75% identical to HYUC_RHIML: N-carbamoyl-L-amino-acid hydrolase (hyuC) from Rhizobium meliloti

KEGG orthology group: K06016, N-carbamoyl-L-amino-acid hydrolase [EC: 3.5.1.87] (inferred from 94% identity to sit:TM1040_1498)

MetaCyc: 75% identical to N-carbamoyl-L-amino-acid hydrolase subunit (Sinorhizobium meliloti CECT4114)
N-carbamoyl-L-amino-acid hydrolase. [EC: 3.5.1.87]

Predicted SEED Role

"Beta-ureidopropionase (EC 3.5.1.6)" in subsystem Hydantoin metabolism or L-2-amino-thiazoline-4-carboxylic acid-Lcysteine conversion or Pyrimidine utilization (EC 3.5.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.6 or 3.5.1.87

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DPL0 at UniProt or InterPro

Protein Sequence (416 amino acids)

>PGA1_c12840 Beta-ureidopropionase (EC 3.5.1.6) (Phaeobacter inhibens DSM 17395)
MTSLGQNLKINGDRLWDSLMEMAKIGPGVAGGNNRQTLTDADAEGRALFQSWCEAAGCTM
GLDTMGNMFARREGTDPDALPVYMGSHLDTQPTGGKYDGVLGVLGGLEVVRTLNDLGIKT
KHPIVVTNWTNEEGTRFAPAMLASGVFAGKHTQDWAYAREDADGKTFGDELKRIGWVGEE
QVGARKMHAMFELHIEQGPILEVENKDIGVVTHGQGLWWLQCTVTGKDAHTGSTPMNMRV
NAGLGMARMTEAAHQIAMAHQPHAVGAVGHCDVYPNSRNVIPGKVVFTVDFRSPDLEKLT
SMRAQYEVKAKEIADELGLGLEIEPVGHFDPVTFDEGCVNAVRSAAERLGYSHMDIVSGA
GHDACWINDLAPTAMIMCPCVGGLSHNEAEDISKDWAAAGTDVMLHAVLETAEIVA