Protein Info for HP15_1239 in Marinobacter adhaerens HP15

Annotation: phosphoribosylformylglycinamidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1301 TIGR01735: phosphoribosylformylglycinamidine synthase" amino acids 4 to 1300 (1297 residues), 2045.7 bits, see alignment E=0 PF18076: FGAR-AT_N" amino acids 35 to 150 (116 residues), 141.2 bits, see alignment E=2.9e-45 PF18072: FGAR-AT_linker" amino acids 171 to 220 (50 residues), 79.9 bits, see alignment (E = 3.2e-26) PF02769: AIRS_C" amino acids 432 to 588 (157 residues), 125 bits, see alignment E=6.5e-40 amino acids 837 to 972 (136 residues), 46.8 bits, see alignment E=7.2e-16 PF13507: GATase_5" amino acids 1045 to 1300 (256 residues), 370.2 bits, see alignment E=9.1e-115

Best Hits

Swiss-Prot: 71% identical to PUR4_HAHCH: Phosphoribosylformylglycinamidine synthase (purL) from Hahella chejuensis (strain KCTC 2396)

KEGG orthology group: K01952, phosphoribosylformylglycinamidine synthase [EC: 6.3.5.3] (inferred from 64% identity to abo:ABO_0785)

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PHN8 at UniProt or InterPro

Protein Sequence (1301 amino acids)

>HP15_1239 phosphoribosylformylglycinamidine synthase (Marinobacter adhaerens HP15)
MLELRGAPALSPFRSRKLHSRIQDIVPEIEHVYAEFMHFVDLEADLLDAEQAILDRLLTY
GPSVPVEEPDGVLFLVVPRPGTLSPWSSKATDIARNCGLRQIHRIERGTAYYIRSGRKLG
LEQREKIAALLHDRMTQKVFHEMGGAELLFSHEEPRLLGRVPVLAGGRDALVEANSRLGL
ALAEDEIDYLVKSFTDLERDPTDVELMMFAQANSEHCRHKIFNASWDIDGEGQEKSLFAM
IRNTFEMNSEGVLSAYKDNASVIRGSRGGRFFPDPETGVYGYNEEDIHILMKVETHNHPT
AIAPAAGAATGSGGEIRDEGATGRGSKPKAGLTGFTVSNLNLPDDPQPWEINYGKPERIA
SPLDIMIEGPIGGAAFNNEFGRPNLAGYFRTFEEKVPGAAGEEVRGYHKPIMIAGGLGNI
REEHVEKGDIPVGAKLIVLGGPSMLIGLGGGAASSMDSGSSNENLDFASVQRDNPEMERR
CQEVIDRCWQMGDKNPIAFIHDVGAGGLSNAMPELVKDGGRGGKFELRDIPSDEPGMSPL
EIWCNESQERYVMAVAPENLEQFDALCRRERCPYAVIGEATEAHHLELGDSYFDDKPVDL
PMEVLFGKPPRMHRSVSRASFTKPIFDSTKIDLDDAIRRVLRLPSVGSKSFLITIGDRTI
TGLVARDQMVGPWQVPVADVAVTATSFDVRTGEAMAMGERTPVATIDAPASGRMAVGEVI
TNMVAAPIGKLSDIRLSANWMAAAGHPGEDENLYETVRAVGMELCPELGITIPVGKDSMS
MKTMWEEDSGEQKSVTAPLSLIVSGFAPVIDVARTLTPQLVKDAGETDLILVDLAAGQNR
LGGSALAQVYRQVGAVAPDLDDPEDIKAFFAVIQGLNSDGKLLAYHDRSDGGLFVTLAEM
CFAGHCGVDIKLDGLAEDRSQFARELFNEELGAVIQVRKQDTGFVLQQFSAAGLGDYISV
IGSPNDTDNLRLTFEGSTVVDESRVELQRLWSETSYRVQSLRDNADCAREEFDNLLDAED
PGLHAELSYDINDDVAAPYINKGVRPKVAVLREQGVNGQVEMAAAFDRAGFDSVDVHMSD
LLSGRVTLDGFNSLVACGGFSYGDVLGAGEGWAKSILFSDRVRDQFAAFFNRQDTLALGV
CNGCQMLSNLHELIPGSEGWPRFVRNQSEQFEARLVMAEVMPSPSAFMDGMAGSRMPIAV
AHGEGRVEFASGTSAEALSENELVALRYVDNRGQATVRYPYNPNGSEAGITGVTTRDGRV
TIMMPHPERVFRAVQHSWRPSEWQEDGSWIRMFRNARRWFG