Protein Info for PGA1_c12530 in Phaeobacter inhibens DSM 17395

Annotation: ATP-dependent Clp protease ATP-binding subunit ClpX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 TIGR00382: ATP-dependent Clp protease, ATP-binding subunit ClpX" amino acids 10 to 410 (401 residues), 663.1 bits, see alignment E=8.7e-204 PF06689: zf-C4_ClpX" amino acids 14 to 50 (37 residues), 70.7 bits, see alignment 2.6e-23 PF07724: AAA_2" amino acids 112 to 308 (197 residues), 120.9 bits, see alignment E=2.1e-38 PF07728: AAA_5" amino acids 114 to 190 (77 residues), 25.1 bits, see alignment E=5.6e-09 PF00004: AAA" amino acids 115 to 232 (118 residues), 62.2 bits, see alignment E=2.5e-20 PF10431: ClpB_D2-small" amino acids 315 to 386 (72 residues), 47.5 bits, see alignment E=5.5e-16

Best Hits

Swiss-Prot: 95% identical to CLPX_RUEST: ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) from Ruegeria sp. (strain TM1040)

KEGG orthology group: K03544, ATP-dependent Clp protease ATP-binding subunit ClpX (inferred from 95% identity to sit:TM1040_1526)

MetaCyc: 68% identical to ATP-dependent Clp protease ATP-binding subunit ClpX (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpX" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7EYI4 at UniProt or InterPro

Protein Sequence (422 amino acids)

>PGA1_c12530 ATP-dependent Clp protease ATP-binding subunit ClpX (Phaeobacter inhibens DSM 17395)
MATNSSGDSKNTLYCSFCGKSQHEVRKLIAGPTVFICDECVELCMDIIREETKASGLKST
DGVPTPKDICEVLDDYVIGQAMAKRVLSVAVHNHYKRLNHAQKAGSDIELSKSNILLIGP
TGCGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDVENIILKLLQASEYNVERAQRGI
VYIDEVDKITRKSENPSITRDVSGEGVQQALLKLMEGTVASVPPQGGRKHPQQEFLQVDT
TNILFICGGAFAGLDKIIKQRGKGSAMGFGADVRDDSDAGVGETFKDLEPEDLLKFGLIP
EFVGRLPVLATLEDLDEDALITILTKPKNALVKQYQRLFELEDTELDFTEEALSSIAKKA
IERKTGARGLRSILEDILLNTMFELPGMDSVEKVVVNEEAVNSDAQPLMIHADAEKESAT
AG