Protein Info for Psest_1247 in Pseudomonas stutzeri RCH2

Annotation: Archaeal fructose-1,6-bisphosphatase and related enzymes of inositol monophosphatase family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 85% identical to SUHB_PSEAE: Inositol-1-monophosphatase (suhB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01092, myo-inositol-1(or 4)-monophosphatase [EC: 3.1.3.25] (inferred from 97% identity to psa:PST_3046)

MetaCyc: 53% identical to Nus factor SuhB (Escherichia coli K-12 substr. MG1655)
Inositol-phosphate phosphatase. [EC: 3.1.3.25]; 3.1.3.25 [EC: 3.1.3.25]; Glycerol-2-phosphatase. [EC: 3.1.3.25, 3.1.3.19]

Predicted SEED Role

"Inositol-1-monophosphatase (EC 3.1.3.25)" in subsystem Di-Inositol-Phosphate biosynthesis (EC 3.1.3.25)

MetaCyc Pathways

myo-inositol biosynthesis (1/2 steps found)
D-myo-inositol (1,4,5)-trisphosphate degradation (1/3 steps found)
superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism (1/6 steps found)
phytate degradation I (3/14 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.25

Use Curated BLAST to search for 3.1.3.19 or 3.1.3.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GK83 at UniProt or InterPro

Protein Sequence (271 amino acids)

>Psest_1247 Archaeal fructose-1,6-bisphosphatase and related enzymes of inositol monophosphatase family (Pseudomonas stutzeri RCH2)
MQPMLNIALRAARSAGELIVRSTDRLDAISVNEKEAKDYVTEIDKAAEQSIVAALRKAYP
NHGILGEEGGLLEGSGDGTDYLWIIDPLDGTTNFVRGIPHYAVSIACKYRGRLEHAVVLD
PVRQEEFTASRGRGAAVNGRRLRVSARKSLDGALLGTGFPFKDGDMDNLDAYLNMFRSLV
GQTSGLRRAGAASLDLAYVAAGRFDAFWEFGLSEWDMAAGALLIQEAGGLVSDFSGGHDF
LEKGQIVAGNTKCFKAVLTAIQPHLTPSLKR