Protein Info for Psest_1227 in Pseudomonas stutzeri RCH2

Annotation: Predicted SAM-dependent methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 330 PF05971: Methyltransf_10" amino acids 17 to 314 (298 residues), 451.3 bits, see alignment E=1.7e-139 PF05175: MTS" amino acids 118 to 210 (93 residues), 33.7 bits, see alignment E=2.9e-12

Best Hits

Swiss-Prot: 80% identical to RLMF_PSEU5: Ribosomal RNA large subunit methyltransferase F (rlmF) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K06970, ribosomal RNA large subunit methyltransferase F [EC: 2.1.1.181] (inferred from 80% identity to psa:PST_3066)

MetaCyc: 56% identical to 23S rRNA m6A1618 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11596 [EC: 2.1.1.181]

Predicted SEED Role

"Ribosomal RNA large subunit methyltransferase F (EC 2.1.1.51)" (EC 2.1.1.51)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.51

Use Curated BLAST to search for 2.1.1.181 or 2.1.1.51

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIE7 at UniProt or InterPro

Protein Sequence (330 amino acids)

>Psest_1227 Predicted SAM-dependent methyltransferase (Pseudomonas stutzeri RCH2)
MPRKSPSPTPQPARAEAKAVLHPRNRHTGRYDFPALIAGCPELAEHVIINPYGKQSIDFA
NPDAVRVFNRALLRQFYGIQHWDIPPGYLCPPVPGRADYLHGLADLLAQNDASAIPRGTA
IHALDIGTGANCIYPLIGHREYGWRFTGSDIDATALASAHTIVSANGLAKAIELRQQAAP
KHIFKGMVLPEDRFDLTLCNPPFHASQAEASSGSQRKWRNLGKLDPSRKLPALNFGGQAA
ELWCEGGEAAFVARMAEESALFAGQVYWFSTLVSKGANVAPLEARLKRLGARQVHTLDMA
QGQKKSRFVAWTFLTEAEQATWRAERWPKG