Protein Info for HP15_113 in Marinobacter adhaerens HP15

Annotation: outer membrane protein, probably efflux family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 406 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF02321: OEP" amino acids 39 to 200 (162 residues), 33.8 bits, see alignment E=1.6e-12 amino acids 234 to 392 (159 residues), 52.6 bits, see alignment E=2.6e-18

Best Hits

KEGG orthology group: None (inferred from 54% identity to kko:Kkor_1262)

Predicted SEED Role

"Heavy metal RND efflux outer membrane protein, CzcC family" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PJ58 at UniProt or InterPro

Protein Sequence (406 amino acids)

>HP15_113 outer membrane protein, probably efflux family (Marinobacter adhaerens HP15)
MMKASKHSPRALLCSVLLSIPLLGQAETVNWANWLTGQISKHPEVLAATEQAAARKEDAQ
ASEQPLYNPELSVGADRTGSENNFEAGLSQTIDWSDQQGALKEKAKLIRLSAQAGLSEAV
LTQTSESLFALVEWRTTTLLEEIAESQQQRLDALLDQVEQRQQAGDLGRADAELLFLNLS
RQLSEVAQAKAAVDRAETQVRERLPDWRPQDGGIPEYIWSALESEIGDADLLAHPSIAAA
RAEWQVLKSEAEVARRKATASPTVGLSGGRDGGENVVGLTFSIPLNVRNNYSAETRAANR
RALEAEARFQALYRKRQYDLAGARASWKRYDKQLRRWKELSQGRVQRSADLLEKQWQVGD
LSTSEYLQALGQRAESLKTGIELEKLAQLGLIELLSQSGELITPFQ