Protein Info for Psest_1123 in Pseudomonas stutzeri RCH2

Updated annotation (from data): adhesin-associated sorting protein (DUF1329)
Rationale: Conserved cofit with a putative adhesin (Psest_1122). DUF1329 is distantly related to lolB, which inserts lipoproteins into the outer membrane. So we predict that DUF1329 is involved in sorting the adhesin to the outer membrane.
Original annotation: Protein of unknown function (DUF1329).

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 453 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF07044: DUF1329" amino acids 82 to 448 (367 residues), 512.7 bits, see alignment E=7.1e-158 PF17131: LolA_like" amino acids 320 to 401 (82 residues), 30.6 bits, see alignment E=2.6e-11

Best Hits

Swiss-Prot: 66% identical to Y3922_PSEAE: Uncharacterized protein PA3922 (PA3922) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 98% identity to psa:PST_3172)

Predicted SEED Role

"FIG002188: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIS7 at UniProt or InterPro

Protein Sequence (453 amino acids)

>Psest_1123 adhesin-associated sorting protein (DUF1329) (Pseudomonas stutzeri RCH2)
MKTTRKLFSVLAFSLLASSVMAAVSPEEAAKLGTSLTPLGAEKAGNADGSIPEWTGGLKT
DAAPLKNGHLTNPFKDEQPKFVITAQNAEQYKDKLTAGQLAMFKRYPETYKIRVFPTHRS
VAVPDEIYQAAKKSAVNTELVEGGNGIANFTDSRYYAFPIPKNGLEVVWNHITRYRGGNV
RRNIVQATPQTNGSYTMVHFEDEVAFPLGMTDLDPERAKNALLFFKQRVTAPSRLAGNVL
LVHDSLDQVKDPRQAWIYNAGQRRVRRAPQVAYDGPGTASDGMRTTDNFDMFSGAPDRYD
WKLVGKKELFIPYNSYEIASSDLKYKDVIKAGHVNQDLARYELHRVWEIEATLKSGERNI
YAKRRFFVDEDSWTIVQSELYDGRGQLWRVGEAHSLQYYQHKVPAYAFEALYDIISGRYI
AIGMSNEEKPHEYGYRASARDFTPAALRNAGVR