Protein Info for HP15_109 in Marinobacter adhaerens HP15

Annotation: cation efflux family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 394 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 62 to 379 (318 residues), 162 bits, see alignment E=8.7e-52 PF16576: HlyD_D23" amino acids 73 to 305 (233 residues), 124.6 bits, see alignment E=5.3e-40 PF13533: Biotin_lipoyl_2" amino acids 89 to 125 (37 residues), 27.6 bits, see alignment 3e-10 PF13437: HlyD_3" amino acids 202 to 301 (100 residues), 60.2 bits, see alignment E=4.4e-20

Best Hits

Predicted SEED Role

"Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PJ54 at UniProt or InterPro

Protein Sequence (394 amino acids)

>HP15_109 cation efflux family protein (Marinobacter adhaerens HP15)
MIRNMQIMFVAISFLVYQPLAHGAEEAEGGQHQKGEAGHSAEGSEAREVHLTPEQRELLS
VQTVTVPRGRADNIVTAPAEVHYVPDRVAEVGPLLQGKLTRLAVDLGDQVEKGQLLAALN
SVELARIRSRLNSLKARKEAATAEYQREKQLRSESFTSEEEFLDAKAAYLEITAEYDSIR
EQLAVFGSDSSGADSSLAQYALRSPIQGRIERMDARLGQTLSSSDTPFTVADTSVVWVML
QVAETDIGRVSVGDEVQVFSKSVEDRFHTGEVSWISNELDEETRTIPVRVVLESPNGDLR
PNTYATARIMTESAASLPLVPHDAVQTIDDESVVFVPGNERGAYKAVPVTLGKEANDWIE
IKSGISANTEVVSVGAFDLMSAITASGRSAAHGH