Protein Info for HP15_1058 in Marinobacter adhaerens HP15

Annotation: 2,4-dienoyl-CoA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 689 PF00724: Oxidored_FMN" amino acids 15 to 339 (325 residues), 308 bits, see alignment E=3.9e-95 PF03486: HI0933_like" amino acids 385 to 424 (40 residues), 26.2 bits, see alignment 1.4e-09 PF07992: Pyr_redox_2" amino acids 385 to 666 (282 residues), 97.6 bits, see alignment E=3.7e-31 PF00070: Pyr_redox" amino acids 385 to 421 (37 residues), 23.8 bits, see alignment 2.1e-08 PF13450: NAD_binding_8" amino acids 388 to 423 (36 residues), 36.9 bits, see alignment 1.5e-12

Best Hits

Swiss-Prot: 63% identical to FADH_ECOLI: 2,4-dienoyl-CoA reductase (fadH) from Escherichia coli (strain K12)

KEGG orthology group: K00219, 2,4-dienoyl-CoA reductase (NADPH2) [EC: 1.3.1.34] (inferred from 89% identity to maq:Maqu_2074)

MetaCyc: 63% identical to 2,4-dienoyl-CoA reductase (Escherichia coli K-12 substr. MG1655)
2,4-dienoyl-CoA reductase (NADPH). [EC: 1.3.1.34]

Predicted SEED Role

"2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34)" (EC 1.3.1.34)

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.34

Use Curated BLAST to search for 1.3.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PG80 at UniProt or InterPro

Protein Sequence (689 amino acids)

>HP15_1058 2,4-dienoyl-CoA reductase (Marinobacter adhaerens HP15)
MTASTTPGVAKYPNLLEPLDLGFTKLRNRTLMGSMHTGLEEAKNGFERLAAFYAERARGG
AGLIVTGGIAPNVEGGVFQHAAKMTTEEEVEKHRVITDAVHGADGKICMQILHAGRYAYS
PELVAPSAIQAPINPFKPRELDEDGIEKQIQDYAECAALAQSAGYDGVEVMGSEGYFINQ
FIVSHTNHRTDRWGGSYENRIRLPIEIVRRVRERVGENFILIYRLSMLDLIEDGSTWEEV
VHLAKEIEKAGATIINTGIGWHEARVPTIATSVPRGAFTKVTARLKGEVSIPLVTTNRIN
MPDVAEKILAEGDADMVSMARPFLADADLVLKAAEDRAEEINTCIGCNQACLDHTFSGKL
TSCLVNPRACHETELTYVKTAKPKSIAVVGAGPAGLAFASVAAERGHKVTLFDAGSEIGG
QFNVAKLIPGKEEFYETLRYFRVMLEKHQVDVRLNTRVSADDLKAGGFDEVILATGVKPR
TPEIEGIDHPKVIGYLDALLERKPVGQKVAVIGAGGIGFDVSEFIVHKGTSAALDTDHFM
REWGVDLSVEHRGGIQGVEPRVPEPAREVYLLQRKASKVGKNLGKTTGWIHRTSLKNRQV
QMVPGVTYRKIDDEGLHITVTPKGAEQGEDRVLPVDTIIVCAGQEPLRELQSELEAAGLP
VHLIGGSDVAAELDAKRAINQGSRLAAEL