Protein Info for Psest_1100 in Pseudomonas stutzeri RCH2

Annotation: Type I site-specific restriction-modification system, R (restriction) subunit and related helicases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 932 PF04313: HSDR_N" amino acids 57 to 123 (67 residues), 32.6 bits, see alignment 2.3e-11 PF04851: ResIII" amino acids 159 to 312 (154 residues), 139.4 bits, see alignment E=3.1e-44 PF00270: DEAD" amino acids 162 to 313 (152 residues), 47.4 bits, see alignment E=5.6e-16 PF18766: SWI2_SNF2" amino acids 163 to 341 (179 residues), 61.4 bits, see alignment E=3.1e-20 PF00271: Helicase_C" amino acids 424 to 524 (101 residues), 25.1 bits, see alignment E=5.3e-09 PF08463: EcoEI_R_C" amino acids 775 to 926 (152 residues), 98.7 bits, see alignment E=1.2e-31

Best Hits

KEGG orthology group: K01153, type I restriction enzyme, R subunit [EC: 3.1.21.3] (inferred from 90% identity to neu:NE2499)

Predicted SEED Role

"Type I restriction-modification system, restriction subunit R (EC 3.1.21.3)" in subsystem Restriction-Modification System (EC 3.1.21.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.3

Use Curated BLAST to search for 3.1.21.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GI31 at UniProt or InterPro

Protein Sequence (932 amino acids)

>Psest_1100 Type I site-specific restriction-modification system, R (restriction) subunit and related helicases (Pseudomonas stutzeri RCH2)
MSKSEAQTRADLIDQQLALSGWNVKDPTQVVEEFDILTALPEGVAEPGTPYQGHQFSDYV
LLGKDGKPLAVIEAKKTLRDAALGREQAKQYCYNIQKQLGSELPFCFYTNGFETYFWDLG
NAPPRKVIGFPTRDDLERFAYIRRNRKPLTQEFINTSIAGRDYQIRAIRSVLEGIEQKKR
DFLLVMATGTGKTRTCIAMVDALMRAGHAERVLFLVDRIALREQALATFKEHLPNEPRWP
NVGEKLIAKDRRIYVATYPTMLNIVRDDVQHLSPHFFDFIVVDESHRSIYNTYGEILDYF
KTITLGLTATPTDIIDHNTFQLFHCEDGLPTFAYTFEEAVNRVPPYLCNFQVMKIQTRFQ
MEGISKRTISLEDQKKLILQGKDIEEINFEGTQLEKQVINKGTNTLIVREFMEECIKDGN
GVLPGKTIFFCSSKAHARRIEEIFDKLYPQYHGELAKVLVSDDPRVYGKGGLLDQFTNND
MPRIAISVDMLDTGIDVREIVNLVFAKPVYSYTKFWQMIGRGTRLLEASKPKPWCTEKDV
FLILDCWDNFEYFKLQPKGKELKPQLPLPVRLVGLRLDKIDKAIAINQIDITEREVAKLR
QQIAALPKDSVVIKDAAVALARLDEENFWINLTPQKLEFLRAEIKPLFRTVSEADFKAMR
FERDVLEYSLAVLNDDKEQADTIKEGIVEQIGELPLSVSFVKQEEALIRAAQASHYWATA
NEDAFDELVAKLGPLMKFREQMASPGPVHLDLTDILHNKERVEFGPQHEAVSITRYREMV
ENLIAELTAHNPVLQKIKNGEPVSSEEANALAELLHEEHPHITEDLLRQVYKNRKARFIQ
FIRHILGIEVLQSFPDEVSAAFDQFIRSHTTLSSRQMEFLNLLKGFIIEREKVEKKDLIN
APFTVIHPQGIRGVFSPLEINEILQLTERLAA