Protein Info for PGA1_c10620 in Phaeobacter inhibens DSM 17395

Annotation: peptide chain release factor 3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 535 TIGR00503: peptide chain release factor 3" amino acids 13 to 532 (520 residues), 650.8 bits, see alignment E=1.4e-199 PF00009: GTP_EFTU" amino acids 17 to 281 (265 residues), 171.4 bits, see alignment E=4.2e-54 TIGR00231: small GTP-binding protein domain" amino acids 21 to 165 (145 residues), 76.4 bits, see alignment E=2.2e-25 PF01926: MMR_HSR1" amino acids 22 to 148 (127 residues), 24.4 bits, see alignment E=6.8e-09 PF22042: EF-G_D2" amino acids 301 to 386 (86 residues), 83.8 bits, see alignment E=1.8e-27 PF03144: GTP_EFTU_D2" amino acids 319 to 385 (67 residues), 38.7 bits, see alignment E=2.7e-13 PF16658: RF3_C" amino acids 394 to 518 (125 residues), 137.4 bits, see alignment E=6.5e-44

Best Hits

Swiss-Prot: 51% identical to RF3_HYDCU: Peptide chain release factor 3 (prfC) from Hydrogenovibrio crunogenus (strain XCL-2)

KEGG orthology group: K02837, peptide chain release factor 3 (inferred from 93% identity to sit:TM1040_1807)

Predicted SEED Role

"Peptide chain release factor 3"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DP22 at UniProt or InterPro

Protein Sequence (535 amino acids)

>PGA1_c10620 peptide chain release factor 3 (Phaeobacter inhibens DSM 17395)
MLDTVPNRPDLPAEIARRRTFAIISHPDAGKTTLTEKFLLYGGAIQMAGQVRAKGEARRT
RSDFMQMEKDRGISVSASAMSFDYANYRFNLVDTPGHSDFSEDTYRTLTAVDAAVMVIDG
AKGVESQTQKLFEVCRLRDLPILTFCNKMDRESRDTFEIIDEIQQNLAIDVTPASWPIGV
GRDFIGCYDMLRDRLELMDRADRNKIAESIEINGLDDPQLAEHVPEHLLEKLLEEVEMAR
ELLPTLDPQSVLEGHMTPIWFGSAINSFGVKELMDGISTYGPEPQPQTAQPRQIAPDETK
VAGFVFKVQANMDPKHRDRVAFVRLASGHFKRGMKLTHVRSKKPMAISNPVLFLASDREL
AEEAWAGDIIGIPNHGQLRIGDTLTEGEALRVSGIPSFAPELLQGVRAGDPMKAKHLEKA
LMQFAEEGAAKVFKPSIGSGFIVGVVGQLQFEVLASRIEMEYGLPVRFEASQFTSARWVN
GDKLALDKFINTNKQHIAYDNDGDVVYLTRLQWDIDRVERDFPDLKLTATKEMMV