Protein Info for Psest_1078 in Pseudomonas stutzeri RCH2
Updated annotation (from data): acetyl-CoA:acetoacetate CoA transferase, A subunit (EC 2.8.3.8)
Rationale: Specifically important for: L-Leucine. Important on tyrosine as well. This role makes sense because of its involvement in acetoacetate catabolism (from tyrosine or leucine). It had been annotated as succinyl-CoA:3-ketoacid-coenzyme A transferase (a broader range of substrates and with succinyl-CoA not acetyl-CoA as donor). Similar to E. coli atoD (46% identical), which has the same activity.
Original annotation: 3-oxoacid CoA-transferase, A subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 76% identical to SCOA_XANCP: Succinyl-CoA:3-ketoacid coenzyme A transferase subunit A (lpsI) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
KEGG orthology group: K01028, 3-oxoacid CoA-transferase subunit A [EC: 2.8.3.5] (inferred from 98% identity to psa:PST_3218)MetaCyc: 62% identical to succinyl-CoA-transferase subunit A (Helicobacter pylori 26695)
3-oxoacid CoA-transferase. [EC: 2.8.3.5]
Predicted SEED Role
"Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5)" in subsystem Catechol branch of beta-ketoadipate pathway or Leucine Degradation and HMG-CoA Metabolism or Protocatechuate branch of beta-ketoadipate pathway or Serine-glyoxylate cycle (EC 2.8.3.5)
MetaCyc Pathways
- ketolysis (3/3 steps found)
- TCA cycle VI (Helicobacter) (7/9 steps found)
- L-glutamate degradation V (via hydroxyglutarate) (7/10 steps found)
- L-glutamate degradation XI (reductive Stickland reaction) (4/7 steps found)
- acetyl-CoA fermentation to butanoate (4/7 steps found)
- 4-aminobutanoate degradation V (3/7 steps found)
- gallate degradation III (anaerobic) (5/11 steps found)
- succinate fermentation to butanoate (2/7 steps found)
KEGG Metabolic Maps
- Benzoate degradation via CoA ligation
- Butanoate metabolism
- Propanoate metabolism
- Synthesis and degradation of ketone bodies
- Valine, leucine and isoleucine degradation
Isozymes
Compare fitness of predicted isozymes for: 2.8.3.5, 2.8.3.8
Use Curated BLAST to search for 2.8.3.5 or 2.8.3.8
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GK00 at UniProt or InterPro
Protein Sequence (231 amino acids)
>Psest_1078 acetyl-CoA:acetoacetate CoA transferase, A subunit (EC 2.8.3.8) (Pseudomonas stutzeri RCH2) MNKIYPSAAHALEGLVEDGMTIAVGGFGLCGIPEQLIAALRDSGKKDLTAISNNAGVDGF GLGLLLETRQISKMVSSYVGENKEFERQYLAGELALEFTPQGTLAEKLRAGGAGIPAFYT KTGYGTLVAEGKETRQFNGEWYVMEESLTADLALVKAWKADKAGNLLFRKTARNFNPLAA MAGEVCVVEVEEIVETGELDPDQIHLPGIYVHRIVHNPNPEKRIEKRTVRS