Protein Info for HP15_1008 in Marinobacter adhaerens HP15

Annotation: acyl-CoA dehydrogenase domain protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 383 PF02771: Acyl-CoA_dh_N" amino acids 8 to 120 (113 residues), 109.6 bits, see alignment E=2.2e-35 PF02770: Acyl-CoA_dh_M" amino acids 124 to 219 (96 residues), 96.1 bits, see alignment E=2.3e-31 PF00441: Acyl-CoA_dh_1" amino acids 231 to 380 (150 residues), 151 bits, see alignment E=5.6e-48 PF08028: Acyl-CoA_dh_2" amino acids 246 to 368 (123 residues), 51 bits, see alignment E=3.7e-17

Best Hits

Swiss-Prot: 37% identical to ACDB_BACSU: Acyl-CoA dehydrogenase (mmgC) from Bacillus subtilis (strain 168)

KEGG orthology group: K00249, acyl-CoA dehydrogenase [EC: 1.3.99.3] (inferred from 91% identity to maq:Maqu_2118)

MetaCyc: 46% identical to (4S)-homoplatensicyl-CoA dehydrogenase (Streptomyces platensis)
1.3.8.-

Predicted SEED Role

"Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)" in subsystem Isoleucine degradation (EC 1.3.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.2, 1.3.99.3

Use Curated BLAST to search for 1.3.99.2 or 1.3.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PFM3 at UniProt or InterPro

Protein Sequence (383 amino acids)

>HP15_1008 acyl-CoA dehydrogenase domain protein (Marinobacter adhaerens HP15)
MTVSVDKEELAMFRDSVIKVLEKEVTPHYEAWEKSGLVPRELWNTLGNAGMLCVDVPEEW
GGIGAPFQFSVVVGEELARMGFGALSTNVMVHSDIVAPYLSHMGNEEQRQQWLPKLVSGE
AVGAIAMTEPGAGSDLQAIRTSAVKDGDEYILNGSKTFITNGQHADMVIVAAKTDPKAGA
RGISLFLVDTSLPGFSKGRNLDKIGQHSGDTSELFFSDMRIPASALLGEEGQGFVYLMKE
LPRERLVIGALGVAAARGSLDLTIAYAQERELFGQKLAQLQNTRFEIARMETDYRVNKAF
VDQCIDQYDLGELDAPTASMAKYSATEMQCRVADGCLQLFGGYGYTTEYPISRAFIDARV
QRIYGGTSEVMKEIIARSVLGKA