Protein Info for HP15_1002 in Marinobacter adhaerens HP15

Annotation: methylcrotonyl-CoA carboxylase, alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 664 PF00289: Biotin_carb_N" amino acids 1 to 110 (110 residues), 158.1 bits, see alignment E=3.7e-50 PF02786: CPSase_L_D2" amino acids 116 to 322 (207 residues), 244.3 bits, see alignment E=3.4e-76 PF02222: ATP-grasp" amino acids 139 to 292 (154 residues), 34.6 bits, see alignment E=5.3e-12 PF07478: Dala_Dala_lig_C" amino acids 140 to 291 (152 residues), 36.8 bits, see alignment E=1.1e-12 PF02785: Biotin_carb_C" amino acids 336 to 443 (108 residues), 133.9 bits, see alignment E=8.6e-43 PF00364: Biotin_lipoyl" amino acids 591 to 656 (66 residues), 64.9 bits, see alignment E=1.7e-21

Best Hits

KEGG orthology group: K01968, 3-methylcrotonyl-CoA carboxylase alpha subunit [EC: 6.4.1.4] (inferred from 84% identity to maq:Maqu_2125)

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4

Use Curated BLAST to search for 6.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PFL7 at UniProt or InterPro

Protein Sequence (664 amino acids)

>HP15_1002 methylcrotonyl-CoA carboxylase, alpha subunit (Marinobacter adhaerens HP15)
MFSKILIANRGEIACRIIQTAHRMGIRVVAVYSEADANARHVAMADEAFHIGPAPSSESY
LRAEKIIEIAKKSGAQAIHPGYGFLSENTGFAESCEANNIVFIGPPSSAIAAMGSKSAAK
AIMEKAGVPLVPGYHGDDQSVETLRAEAEKCGFPLLLKAVAGGGGKGMRVVNNMGEFDDA
LAAAKREAQNAFGNPDMLIERYLTQPRHVEIQVFCDQDGKGVYLAERDCSVQRRHQKVLE
EAPAPGLSEETRKAMGEAAVRAAQAINYVGAGTVEFLYDVDGSFFFMEMNTRLQVEHPVT
EMVTGQDLVEWQLKVAWGDALPLEQSQVKTRGHALEARIYAEDPDQDFLPATGNLRYLST
PDESAHVRVDTGVTEGDDISIHYDPMIAKLIVWDETRDQAINRMVQALEHYRIAGVKTNI
RFLHALADAQPFREAELTTGFIDDHRELLFPKSKLDTHKALVLAAGFVLEQRKSREVVST
DPWSPFGRQNSWRMNSEFAQPLQLQVGEEIHDLKVLERDDRYQVFVGDSVYNLTARLDDD
YLQAVINGHRISIHGNLHNDQLMLFYEGDTFNCTVYKESYGFEEMASEGSLAAPMNGAIV
AVQAKVGDKVTAGQSLVIMEAMKMEHAIKAPADGVVTEIFFAEGDQVSEGAELIAIEVNE
EEAG