Protein Info for PGA1_c10240 in Phaeobacter inhibens DSM 17395

Annotation: bifunctional enzyme GlmU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01128: IspD
  • amino acids 4 to 238 (235 residues), 39.6 bits, E=1.3e-13
• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 4 to 434 (431 residues), 513.3 bits, E=2.9e-158
• PF00483: NTP_transferase
  • amino acids 6 to 161 (156 residues), 45.9 bits, E=1.4e-15
• PF12804: NTP_transf_3
  • amino acids 6 to 130 (125 residues), 71.3 bits, E=2.7e-23
• PF00132: Hexapep
  • amino acids 264 to 296 (33 residues), 27.8 bits, see alignment (E = 3.8e-10)
• PF14602: Hexapep_2
  • amino acids 377 to 411 (35 residues), 23.6 bits, 8.9e-09

Best Hits

Swiss-Prot: 78% identical to GLMU_RUEST: Bifunctional protein GlmU (glmU) from Ruegeria sp. (strain TM1040)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 78% identity to sit:TM1040_0728)

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (11/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (5/9 steps found)
• peptidoglycan recycling I (8/14 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (14/33 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (6/24 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Sequence Analysis Tools

See I7EVE5 at UniProt or InterPro

Protein Sequence (451 amino acids)

>PGA1_c10240 bifunctional enzyme GlmU (Phaeobacter inhibens DSM 17395)
MSTALVILAAGKGTRMNSDLPKVLHPIAHAPMLEHALSAGRALTPERTIVVAGHGADYVR
SMMADIDEDAEIVVQEEQLGTAHAVAQTRDALSDFDGDIVVLYGDTPFVSAETLQRMLAA
RQSADLVVLGFTATDPARYGRLLMDGNSLERIVEYKDATEAEREIDFCNSGLMAGNAAQM
FALIDQVDSANASGEYYLTDLVELARADGLQVTAVSCDEAETLGINSRADLAEADRVFQQ
RKRADLMADGVTLMDPKTVYLAFDTVIGRDTIIEPNVVFGPEVTVESGVFIRSFSHFEGC
HISRGSKVGPYARLRPGAELAENTHIGNFVEIKNAEIAEGAKVNHLSYIGDASVGAETNI
GAGTITCNYDGVMKHRTEIGARAFIGSNTMLVAPVRIGDEAMTATGAVVTKSVEDGALAI
ARAEQKNKLGRARKLMDMLRAKKARLSKGAE