Protein Info for PGA1_c10170 in Phaeobacter inhibens DSM 17395

Annotation: putative hippurate hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 387 TIGR01891: amidohydrolase" amino acids 15 to 372 (358 residues), 347.5 bits, see alignment E=4.7e-108 PF01546: Peptidase_M20" amino acids 76 to 382 (307 residues), 170.6 bits, see alignment E=4.4e-54 PF07687: M20_dimer" amino acids 189 to 281 (93 residues), 35 bits, see alignment E=1.2e-12

Best Hits

Swiss-Prot: 43% identical to ILL3_ORYSJ: IAA-amino acid hydrolase ILR1-like 3 (ILL3) from Oryza sativa subsp. japonica

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 91% identity to sit:TM1040_0719)

MetaCyc: 41% identical to beta-tabtoxin peptidase (Pseudomonas syringae)
3.4.11.-

Predicted SEED Role

"Hippurate hydrolase (EC 3.5.1.32)" (EC 3.5.1.32)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.32

Use Curated BLAST to search for 3.5.1.32

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7EXY3 at UniProt or InterPro

Protein Sequence (387 amino acids)

>PGA1_c10170 putative hippurate hydrolase (Phaeobacter inhibens DSM 17395)
MPVKNRFAELQSEVTAWRRDIHENPEILFETHRTSALVAEKLKEFGCDEIVTGIGRTGVV
GVIKGKSDSSGKVIGLRADMDALPIHEQTGLEYASKTDGAMHACGHDGHTAMLLGAAKYL
SETRNFDGTVVVIFQPAEEGGGGGKEMCDDGMMDRWNIQEVYGMHNWPGRPVGSFAIRPG
AFFAATDQFDITFEGRGGHAAKPQETIDTTVMSAQAVLALQTIASRNADPVHQIVVSVTS
FETSSKAFNVIPQSVHIKGTVRTMSGEMRDLAEKRINEICDGIAATFGGTADVTYHRGYP
VMVNHDEQTEFAAKVARDISGGCDDAPLVMGGEDFAFMLEERPGAYILVGNGDTAAVHHP
EYNFNDEAIPAGCSWWAEIVEQRMPAA