Protein Info for EX31_RS24855 in Rahnella sp. WP5

Annotation: 16S rRNA (cytosine(1407)-C(5))-methyltransferase RsmF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 505 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF17125: Methyltr_RsmF_N" amino acids 30 to 127 (98 residues), 90.3 bits, see alignment E=1.9e-29 TIGR00446: NOL1/NOP2/sun family putative RNA methylase" amino acids 63 to 332 (270 residues), 341.2 bits, see alignment E=1.8e-106 PF01189: Methyltr_RsmB-F" amino acids 142 to 331 (190 residues), 175.2 bits, see alignment E=2.7e-55 PF21150: YebU_pre-PUA_dom" amino acids 351 to 428 (78 residues), 122.6 bits, see alignment E=9.5e-40 PF13636: Methyltranf_PUA" amino acids 444 to 492 (49 residues), 43 bits, see alignment 7.7e-15

Best Hits

Swiss-Prot: 75% identical to RSMF_SERP5: Ribosomal RNA small subunit methyltransferase F (rsmF) from Serratia proteamaculans (strain 568)

KEGG orthology group: K11392, ribosomal RNA small subunit methyltransferase F [EC: 2.1.1.-] (inferred from 100% identity to rah:Rahaq_2855)

MetaCyc: 69% identical to 16S rRNA m5C1407 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11593 [EC: 2.1.1.178]

Predicted SEED Role

"Ribosomal RNA small subunit methyltransferase F (EC 2.1.1.-)" (EC 2.1.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.178

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (505 amino acids)

>EX31_RS24855 16S rRNA (cytosine(1407)-C(5))-methyltransferase RsmF (Rahnella sp. WP5)
MLNSAAENLFSSGTALLSKLTTASSFADKLPAAFLEATRAIMPASLSMEDFIAACQRPLR
PSLRVNTLKISVEDFLTLAGSYQWDLEPVPWCTEGFWINRHALDESRLGNALSHLAGLFY
IQEASSMLPVTALFADGTQPTLVMDVAAAPGSKTTQIAALMENQGGIVANEYSASRVKVL
HANISRCGVSNTALTHFDGRVFGAALPETFDAILLDAPCSGEGVIRKDPDAMSNWSENSV
SEIAATQRELIDSAFHALKPGGVLVYSTCTLNAQENQQVCQWLLETYADAVESESLADLF
PGARKAVTQEGYLHVFPQVYDSEGFFVARFRKTDSVPRLPVPGYKVGRFPFSPLSRQDSA
EIIKAAGQSGLLWQEKQYTLWQRDKEIWIFPAVLEPLFGKVRFSRIGIRLAERFPKGFRW
QHEAVVAFSDTETKNRFEIDDELAQSWFRGVDIYPQTAPPADECLVTWLGQPLGLAKRIG
SRIKNSLPRELVRDGAVRPQTLSND