Protein Info for EX31_RS24820 in Rahnella sp. WP5

Annotation: amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 392 TIGR01891: amidohydrolase" amino acids 19 to 377 (359 residues), 371.8 bits, see alignment E=1.9e-115 PF01546: Peptidase_M20" amino acids 78 to 387 (310 residues), 163.7 bits, see alignment E=5.5e-52 PF07687: M20_dimer" amino acids 192 to 289 (98 residues), 52.4 bits, see alignment E=4.7e-18

Best Hits

Swiss-Prot: 40% identical to YTNL_BACSU: Uncharacterized hydrolase YtnL (ytnL) from Bacillus subtilis (strain 168)

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 100% identity to rah:Rahaq_2863)

MetaCyc: 46% identical to beta-tabtoxin peptidase (Pseudomonas syringae)
3.4.11.-

Predicted SEED Role

"N-acetyl-L,L-diaminopimelate deacetylase (EC 3.5.1.47)" in subsystem Lysine Biosynthesis DAP Pathway (EC 3.5.1.47)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.32, 3.5.1.47

Use Curated BLAST to search for 3.5.1.32 or 3.5.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (392 amino acids)

>EX31_RS24820 amidohydrolase (Rahnella sp. WP5)
MNNKSLCTIADVADLVPQLRQVRQHLHQHPELSNEESATAALVAEKLESLGYQVTTAVGG
YGVVGSMKHGNGSRSIGIRADMDALPITERTDLSYSSQFPGKMHACGHDGHTTMLLGAAE
QLARSKNFSGTVHLIFQPAEEIGFNSGAERMLAEQLFERFPCDAVYGLHNHPGYPVGKMM
FRSGPFMAACDTVNITIHGKGGHAARPHMTVDPILVASSLVIALQSVISRNIDPNDTAVV
TIGSLHSGHAANVIPETARLEMSVRSFDPEVRKTLEQRIRTLAENHAAGYGARAEIEYVP
GYPVLVNHDQETAFAVEVAKELLGEENVVDNLPPISGSEDFAYFLQQKPGCFLRLGNGDS
AVLHNPAYNFNDDSLSFGTAYWTRLVERYLVD