Protein Info for EX31_RS24565 in Rahnella sp. WP5
Annotation: adenylosuccinate lyase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 88% identical to PUR8_ECOL6: Adenylosuccinate lyase (purB) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
KEGG orthology group: K01756, adenylosuccinate lyase [EC: 4.3.2.2] (inferred from 100% identity to rah:Rahaq_2924)MetaCyc: 88% identical to adenylosuccinate lyase (Escherichia coli K-12 substr. MG1655)
Adenylosuccinate lyase. [EC: 4.3.2.2]; 4.3.2.2 [EC: 4.3.2.2]
Predicted SEED Role
"Adenylosuccinate lyase (EC 4.3.2.2)" in subsystem De Novo Purine Biosynthesis or Purine conversions (EC 4.3.2.2)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
- superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
- superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
- superpathway of purine nucleotide salvage (13/14 steps found)
- superpathway of adenosine nucleotides de novo biosynthesis II (7/7 steps found)
- inosine-5'-phosphate biosynthesis I (6/6 steps found)
- inosine-5'-phosphate biosynthesis II (5/5 steps found)
- superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
- adenosine ribonucleotides de novo biosynthesis (3/3 steps found)
- inosine-5'-phosphate biosynthesis III (5/6 steps found)
- dZTP biosynthesis (3/5 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Biosynthesis of alkaloids derived from histidine and purine
- Purine metabolism
Isozymes
No predicted isozymesUse Curated BLAST to search for 4.3.2.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (456 amino acids)
>EX31_RS24565 adenylosuccinate lyase (Rahnella sp. WP5) MELSSLTAVSPVDGRYGDKVSALRTIFSEFGLLKFRVQVEVRWLQKLAACAEIKEVPAFD ADANAFLDQIVANFDVNDAQRIKDIEKTTNHDVKAVEYFLKEKVATLPALHAVSEFIHFA CTSEDINNLSHALMLQTARQDVVLPYWKQIIDAVKDLAHKYRDIPLLSRTHGQPATPSTI GKELANVAYRMDRQFKQLQNVEILGKINGAVGNYNAHIVAYPEVNWHQFSEEFVTSLGIT WNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDIWGYIALNHFKQRTIAGEIGSSTMPH KVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQRDLTDSTVLRNLGVGLGYALIAYQAT MKGVSKLEVNEANLANELDHNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDAAGMQ AFIDSLELPEDEKVRLKAMTPGNYIGRATTMVDELK