Protein Info for EX31_RS24445 in Rahnella sp. WP5

Annotation: beta-ketoacyl-ACP synthase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03150: beta-ketoacyl-acyl-carrier-protein synthase II
  • amino acids 4 to 411 (408 residues), 648 bits, E=2.4e-199
• PF00109: ketoacyl-synt
  • amino acids 4 to 247 (244 residues), 209.5 bits, E=6.8e-66
• PF02801: Ketoacyl-synt_C
  • amino acids 255 to 369 (115 residues), 141.5 bits, E=1.2e-45

Best Hits

Swiss-Prot: 89% identical to FABF_ECO57: 3-oxoacyl-[acyl-carrier-protein] synthase 2 (fabF) from Escherichia coli O157:H7

KEGG orthology group: K09458, 3-oxoacyl-[acyl-carrier-protein] synthase II [EC: 2.3.1.179] (inferred from 100% identity to rah:Rahaq_2953)

MetaCyc: 89% identical to 3-oxoacyl-[acyl carrier protein] synthase 2 (Escherichia coli K-12 substr. MG1655)
Beta-ketoacyl-acyl-carrier-protein synthase II. [EC: 2.3.1.179, 2.3.1.41]; 2.3.1.179,2.3.1.41 [EC: 2.3.1.179, 2.3.1.41]; 2.3.1.179,2.3.1.41 [EC: 2.3.1.179, 2.3.1.41]; 2.3.1.179 [EC: 2.3.1.179, 2.3.1.41]

Predicted SEED Role

"3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.179)" (EC 2.3.1.179)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (40/53 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (16/20 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (13/16 steps found)
• superpathway of fatty acid biosynthesis initiation (5/5 steps found)
• biotin biosynthesis I (12/15 steps found)
• (5Z)-dodecenoate biosynthesis I (5/6 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (7/9 steps found)
• cis-vaccenate biosynthesis (4/5 steps found)
• octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (9/12 steps found)
• palmitate biosynthesis III (21/29 steps found)
• fatty acid biosynthesis initiation (mitochondria) (3/4 steps found)
• gondoate biosynthesis (anaerobic) (3/4 steps found)
• 8-amino-7-oxononanoate biosynthesis I (8/11 steps found)
• oleate biosynthesis IV (anaerobic) (10/14 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (22/31 steps found)
• (5Z)-dodecenoate biosynthesis II (4/6 steps found)
• stearate biosynthesis II (bacteria and plants) (4/6 steps found)
• stearate biosynthesis IV (4/6 steps found)
• fatty acid elongation -- saturated (3/5 steps found)
• superpathway of fatty acid biosynthesis II (plant) (30/43 steps found)
• tetradecanoate biosynthesis (mitochondria) (17/25 steps found)
• petroselinate biosynthesis (2/6 steps found)
• anteiso-branched-chain fatty acid biosynthesis (18/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (18/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (18/34 steps found)
• streptorubin B biosynthesis (15/34 steps found)
• mycolate biosynthesis (21/205 steps found)
• superpathway of mycolate biosynthesis (22/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.179, 2.3.1.41

Use Curated BLAST to search for 2.3.1.179 or 2.3.1.41

Protein Sequence (413 amino acids)

>EX31_RS24445 beta-ketoacyl-ACP synthase II (Rahnella sp. WP5)
MSKRRVVVTGLGMLSPVGNTVESTWNALLAGQSGISLIDHFDTSAYATRFAGLVKDFNCE
DFIPRKDARKMDAFIQYGITAGIQAMQDSGLEVTAENAPRIGAAIGSGIGGLGLIEENHS
SLVNGGPRKISPFFVPSTIVNMIAGHLSIMFGLRGPSISIATACTSGVHNIGHAARIIAY
NDADVMLAGGAEKASTPLGVGGFGAARALSTRNDNPQAASRPWDKDRDGFVLGDGAGIMV
LEEYEHARKRGAKIYAEIVGFGMSSDAYHMTSPPEDGAGAALAMENALRDAGVSAAQIGY
INAHGTSTAAGDKAEAQAVKSVFGADAHKVMVSSTKSMTGHLLGAAGAVESIFTLLALRD
QAVPPTINLDNPDEGCDLDFVPHEARQVKDMQYSLCNSFGFGGTNGSLVFRKI