Protein Info for EX31_RS24350 in Rahnella sp. WP5

Annotation: efflux RND transporter periplasmic adaptor subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 380 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 33 to 366 (334 residues), 271.4 bits, see alignment E=4.4e-85 PF16576: HlyD_D23" amino acids 57 to 282 (226 residues), 44.4 bits, see alignment E=1.8e-15 PF13533: Biotin_lipoyl_2" amino acids 59 to 105 (47 residues), 38.4 bits, see alignment 1.3e-13

Best Hits

Swiss-Prot: 35% identical to BEPF_BRUSU: Efflux pump periplasmic linker BepF (bepF) from Brucella suis biovar 1 (strain 1330)

KEGG orthology group: None (inferred from 99% identity to rah:Rahaq_2974)

Predicted SEED Role

"Probable RND efflux membrane fusion protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (380 amino acids)

>EX31_RS24350 efflux RND transporter periplasmic adaptor subunit (Rahnella sp. WP5)
MFPAMSRFMAVPVLLTTLLFPLTRVAAQPLPTVTTATIATQPDGVSRTYLGRLEAVQAVN
VTSRTEGFIARRAFEEGQFVNAGDVLYEIDPALHQAAVAQAKAQLDSANATARHAQTHLN
RIQRLGDARTVSQSDVDTALAARDTARAAVAQAQAALKTQELQLSYTRITAPIAGRIGVS
HFHTGSLVNPASGALTEIVQLDPVRVVISVNERDVLSASHSSGTLEDAFSADNMTLSLRL
SDGSPYMKAPVLESLGNRIDAQTGTLPVRLTVANPDHRLLPGGTVNVAAEPRSGHSLPVL
PAQALQQNAAGHFVLLVNDGQRVEARPVRLGAQTGHGYFVIEGLQGGEKVVTEGLQWIRP
GMEVNARAATDAPDMAAKKR