Protein Info for EX31_RS23490 in Rahnella sp. WP5

Annotation: cell envelope integrity protein TolA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 411 transmembrane" amino acids 16 to 35 (20 residues), see Phobius details TIGR02794: protein TolA" amino acids 14 to 202 (189 residues), 127.2 bits, see alignment E=4.5e-41 amino acids 194 to 410 (217 residues), 162.9 bits, see alignment E=6.4e-52 PF06519: TolA" amino acids 315 to 408 (94 residues), 119.2 bits, see alignment E=3.8e-39

Best Hits

Swiss-Prot: 56% identical to TOLA_ECOLI: Tol-Pal system protein TolA (tolA) from Escherichia coli (strain K12)

KEGG orthology group: K03646, colicin import membrane protein (inferred from 88% identity to rah:Rahaq_3138)

Predicted SEED Role

"TolA protein" in subsystem Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (411 amino acids)

>EX31_RS23490 cell envelope integrity protein TolA (Rahnella sp. WP5)
MVKATATEQNDKLKRAVIVSVVLHIIIIALLIWGSLDEDTSMSGGGGGSDISAVMVDPSA
VVDQYNRQQQQQADSQRAAAARQKKSEQQAEELQQKQAAEQQRLKELEKERLQAQENAKQ
QAQEQAQQQKQAEQAAEQAKEQQKQAEAAAAQAKAEAAKAAAQAKADAAKEAAKAQADAQ
KKAAADAKKQAEAEAAAAAAAAKKQAEADAKKEAAAEAKKQAAAEAKAQAAADAKAQADA
DAKAAADAKAAAAAEKKAEAAAAAKKAAADKKAAAAAAAKEAADANNLFDGLADSKNAPK
GATGGGASAPSGKGTQKKAGASGADIANYGSQIKAAIESRFYDASSYTGKTCALRVKMNP
DGSLISVSAEGGDPALCQAALAAARQAKFPKPPSEAVYEVFKNAPIDFKPQ