Protein Info for EX31_RS23485 in Rahnella sp. WP5

Annotation: colicin uptake protein TolR

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 143 transmembrane" amino acids 20 to 40 (21 residues), see Phobius details PF02472: ExbD" amino acids 11 to 142 (132 residues), 78.9 bits, see alignment E=1.9e-26 TIGR02801: protein TolR" amino acids 14 to 141 (128 residues), 132 bits, see alignment E=9.8e-43

Best Hits

Swiss-Prot: 82% identical to TOLR_SHIFL: Tol-Pal system protein TolR (tolR) from Shigella flexneri

KEGG orthology group: K03560, biopolymer transport protein TolR (inferred from 100% identity to rah:Rahaq_3139)

Predicted SEED Role

"Tol biopolymer transport system, TolR protein" in subsystem Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (143 amino acids)

>EX31_RS23485 colicin uptake protein TolR (Rahnella sp. WP5)
MARSRGRRNRREGKSEINIVPLLDVLLVLLLIFMATAPIITQSVEVDLPDATDSKTVSSD
DNPPVIVEVSGVGQYTIVVDHQRMEQLPEQQVIAEATSRIQANPKTVFLIGGAKDVPYDE
IIKALNMLHQAGVKSVGLMTQPI