Protein Info for EX31_RS23405 in Rahnella sp. WP5

Annotation: endonuclease VIII

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01149: Fapy_DNA_glyco
  • amino acids 1 to 104 (104 residues), 48 bits, E=2.7e-16
• PF06831: H2TH
  • amino acids 127 to 209 (83 residues), 46.9 bits, E=3.4e-16
• PF06827: zf-FPG_IleRS
  • amino acids 236 to 263 (28 residues), 24.7 bits, see alignment (E = 2.5e-09)

Best Hits

Swiss-Prot: 70% identical to END8_CROS8: Endonuclease 8 (nei) from Cronobacter sakazakii (strain ATCC BAA-894)

KEGG orthology group: K05522, endonuclease VIII [EC: 3.2.2.- 4.2.99.18] (inferred from 100% identity to rah:Rahaq_3156)

MetaCyc: 68% identical to endonuclease VIII (Escherichia coli K-12 substr. MG1655)
RXN-21250

Predicted SEED Role

"Endonuclease VIII" in subsystem DNA Repair Base Excision

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.-, 4.2.99.18

Use Curated BLAST to search for 3.2.2.- or 4.2.99.18

Protein Sequence (264 amino acids)

>EX31_RS23405 endonuclease VIII (Rahnella sp. WP5)
MPEGPEIRRAADVLATAVVNKPLTDVWFAFPALKPYQSALRGTRITSIETRGKALLTHFS
NGLTLYSHNQLYGVWRVVNAGEVAKETKRSLRVKLETTEAAILLYSASEISIYDTAEIEK
HPFLQRIGPDVLDERLTVAQVRERLLSPKFCNRQLGGMLLDQAFLAGLGNYLRAEILWLA
KLLPDHKPKELNDTERDALADACLSVARLSYATRGTMDENVHHGALFRFKVFGRTGQPCE
RCGDPIMKTSVSSRPFFWCPVCQV