Protein Info for EX31_RS22765 in Rahnella sp. WP5

Annotation: tRNA(Met) cytidine acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 661 PF08351: TmcA_N" amino acids 56 to 148 (93 residues), 28.6 bits, see alignment E=5.2e-10 PF13604: AAA_30" amino acids 174 to 282 (109 residues), 28.6 bits, see alignment E=4.4e-10 PF05127: Helicase_RecD" amino acids 193 to 339 (147 residues), 135.6 bits, see alignment E=7e-43 PF13718: GNAT_acetyltr_2" amino acids 373 to 475 (103 residues), 32.7 bits, see alignment E=2.1e-11 amino acids 480 to 538 (59 residues), 27 bits, see alignment 1.1e-09 PF13673: Acetyltransf_10" amino acids 445 to 523 (79 residues), 27.8 bits, see alignment E=8.8e-10 PF13508: Acetyltransf_7" amino acids 459 to 515 (57 residues), 27.6 bits, see alignment 1.2e-09 PF00583: Acetyltransf_1" amino acids 461 to 497 (37 residues), 22.7 bits, see alignment 4.1e-08 PF17176: tRNA_bind_3" amino acids 542 to 656 (115 residues), 140.8 bits, see alignment E=1e-44

Best Hits

Swiss-Prot: 55% identical to TMCA_PECAS: tRNA(Met) cytidine acetyltransferase TmcA (tmcA) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K06957, tRNA(Met) cytidine acetyltransferase [EC: 2.3.1.-] (inferred from 97% identity to rah:Rahaq_3259)

Predicted SEED Role

"Predicted P-loop ATPase fused to an acetyltransferase COG1444"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-

Use Curated BLAST to search for 2.3.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (661 amino acids)

>EX31_RS22765 tRNA(Met) cytidine acetyltransferase (Rahnella sp. WP5)
MSATLLQQQMQRRGVRRLLVFSGGTQWCEQQAVLFSAQSAGDWLWLSDSPPDGVTSCPPS
VAQTLLGQDIFHGVFDARYGLNTEALAVFSGTLKAGSWLILLIPEWNAWPGTPDADSLRW
SEQPQAIPTPRFIRRFRSLLEQDRNITVCRQDQPFDIPPLPAAPDWLPPDGQPTAEQSQI
LQQLMQAQSGVWVLTAARGRGKSTLAGMLARQWPGTCWVTAPAKTSACQILNHQTESARF
IAPDALLSLCREGAVTDIDWLIIDEAAAIPTPMLSEIIAAFPRVLLTTTVQGYEGTGRGF
LLKFCASLPRWHDLRLNSPIRWSSSDTLEPFISQLLLFDEHLPVAEQTGGTSEIFSIDAD
DWLSEPALLEDFYGLLTSAHYRTSPLDLRRLLDAPGMTFSASRQKNTLTGALWLVDEGGL
SAQLAQEVWAGRRRPRGNLVAQSLAAHAGFPEAAVMRSRRVSRIAVHPAFRRRGIARDLL
AAQKQQARQAGLDFLSVSFGYTSDLASLWQQAGFRLVRVSMQKEASSGCYAAMAILPLSA
AGQALADRAVHQLDRDWRWLAPQMDLTLPLDTTADTQPDDADWRELNGFAFAHRPLESSL
PALSRLLLHSALPLTALRAHLQQGQSVAQVVMSLKLSGRKALVSRWREETRQALKAAGMA
E