Protein Info for EX31_RS22675 in Rahnella sp. WP5

Annotation: exopolyphosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 514 TIGR03706: exopolyphosphatase" amino acids 14 to 308 (295 residues), 334.3 bits, see alignment E=3.3e-104 PF02541: Ppx-GppA" amino acids 28 to 310 (283 residues), 331.7 bits, see alignment E=4.2e-103 PF21447: Ppx-GppA_III" amino acids 316 to 492 (177 residues), 205.4 bits, see alignment E=6e-65

Best Hits

Swiss-Prot: 76% identical to PPX_SALTY: Exopolyphosphatase (ppx) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K01524, exopolyphosphatase / guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase [EC: 3.6.1.11 3.6.1.40] (inferred from 100% identity to rah:Rahaq_3278)

MetaCyc: 75% identical to exopolyphosphatase (Escherichia coli K-12 substr. MG1655)
Exopolyphosphatase. [EC: 3.6.1.11]

Predicted SEED Role

"Exopolyphosphatase (EC 3.6.1.11)" in subsystem Phosphate metabolism (EC 3.6.1.11)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.11, 3.6.1.40

Use Curated BLAST to search for 3.6.1.11 or 3.6.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (514 amino acids)

>EX31_RS22675 exopolyphosphatase (Rahnella sp. WP5)
MPLKSSPLNAKPQEIAAIDLGSNSFHMVIARVVNGALQVLGRLKQRVHLADGLDSKNVLS
EEAIQRGLDCLALFAERLQGFPENNVTIVGTHTLRQAVNAEAFLKRAKEVIPYPIEIISG
QEEARLIFMGVEHTQPEKGRKLVVDIGGGSTELVIGEDFEPLLAESRRMGCVSFAQMFFP
NGEISRSNFKRAQLAAAQKLETLAWQYRLQGWQYALGASGSIKAAHEVLVAMGEKDGLIT
PERLEMLSEAVLNFKHFSALDLPGLSEDRQSVFVPGLAILRGVFDALAIKELRLSDGALR
EGVLYEMEGRFRHQDIRTRTAKSLAEHYNIDREQARRVLDTTEQLYAQWLAQNTKLVQPQ
LESLLKFSAMLHEVGLSINHTGMHRHSSYILQNTNLPGFNQEQQTLLATLVRFHRKAIKL
EELPRLNLFKKKHYIPLIQLLRLGALLNNQRQSTTTPESLRLSTDDNHWTLRFPAGYLAQ
NNLVQLDFEREQSYWDDVTGWKLMLEEEDGIVKN