Protein Info for EX31_RS22220 in Rahnella sp. WP5

Annotation: cytochrome o ubiquinol oxidase subunit II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01433: ubiquinol oxidase, subunit II
  • amino acids 13 to 239 (227 residues), 380.7 bits, E=1e-118
• PF00116: COX2
  • amino acids 148 to 218 (71 residues), 30.8 bits, E=2.5e-11
• PF06481: COX_ARM
  • amino acids 239 to 284 (46 residues), 47.3 bits, 1.5e-16

Best Hits

Swiss-Prot: 68% identical to CYOA_ECOL6: Cytochrome bo(3) ubiquinol oxidase subunit 2 (cyoA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K02297, cytochrome o ubiquinol oxidase subunit II [EC: 1.10.3.-] (inferred from 100% identity to rah:Rahaq_3357)

MetaCyc: 68% identical to cytochrome bo3 subunit 2 (Escherichia coli K-12 substr. MG1655)
RXN-21817 [EC: 7.1.1.3]

Predicted SEED Role

"Cytochrome O ubiquinol oxidase subunit II (EC 1.10.3.-)" in subsystem Terminal cytochrome O ubiquinol oxidase or Terminal cytochrome oxidases (EC 1.10.3.-)

MetaCyc Pathways

• D-lactate to cytochrome bo oxidase electron transfer (1/2 steps found)
• NADH to cytochrome bo oxidase electron transfer I (1/2 steps found)
• NADH to cytochrome bo oxidase electron transfer II (1/2 steps found)
• glycerol-3-phosphate to cytochrome bo oxidase electron transfer (1/2 steps found)
• proline to cytochrome bo oxidase electron transfer (1/2 steps found)
• pyruvate to cytochrome bo oxidase electron transfer (1/2 steps found)
• succinate to cytochrome bo oxidase electron transfer (1/2 steps found)

Isozymes

Compare fitness of predicted isozymes for: 1.10.3.-

Use Curated BLAST to search for 1.10.3.- or 7.1.1.3

Protein Sequence (319 amino acids)

>EX31_RS22220 cytochrome o ubiquinol oxidase subunit II (Rahnella sp. WP5)
MRLKKYNKSIGMLSLFASAVMLSGCNTALMDPKGAIGLEQRTLILTAIGLMLIVVIPVII
MAFAFAWKYRASNTKAKYSPDWSHSNKIEAVVWTVPIIIIAILATITWKTTHSLDPFKPI
VTDQKPMTVEVVSLDWKWLFIYPEQGIATVNELVIPKDVPVQFKVTSDSVMNSFFIPQLG
GQIYAMAGMQTQLHLIANEAGTYKGISASFSGRGFSGMKFNTIATPTRADFDTWVAKVKS
APNQLATTDDFNKLAAQSIDNPVEYFSVAKPGLFKEIIGKYSMHDMKAAPEGHTMPMPAG
ADMKGMDMGGQSHAAGAEE