Protein Info for EX31_RS22170 in Rahnella sp. WP5

Annotation: tRNA 4-thiouridine(8) synthase ThiI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 482 TIGR00342: tRNA sulfurtransferase ThiI" amino acids 4 to 376 (373 residues), 433.1 bits, see alignment E=9.1e-134 PF02926: THUMP" amino acids 43 to 161 (119 residues), 82.1 bits, see alignment E=6.5e-27 PF02568: ThiI" amino acids 175 to 368 (194 residues), 264 bits, see alignment E=1.2e-82 TIGR04271: thiazole biosynthesis domain" amino acids 383 to 482 (100 residues), 131.8 bits, see alignment E=8.1e-43 PF00581: Rhodanese" amino acids 409 to 480 (72 residues), 25.8 bits, see alignment E=1.8e-09

Best Hits

Swiss-Prot: 82% identical to THII_PECAS: tRNA sulfurtransferase (thiI) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K03151, thiamine biosynthesis protein ThiI (inferred from 100% identity to rah:Rahaq_3369)

MetaCyc: 78% identical to tRNA uridine 4-sulfurtransferase (Escherichia coli K-12 substr. MG1655)
tRNA sulfurtransferase. [EC: 2.8.1.4]; 2.8.1.- [EC: 2.8.1.4]

Predicted SEED Role

"Thiamine biosynthesis protein thiI"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (482 amino acids)

>EX31_RS22170 tRNA 4-thiouridine(8) synthase ThiI (Rahnella sp. WP5)
MKFIIKLFPEITIKSQSVRLRFIKILSSSIRNIVRPHDETLAVVRHWDHIEVRSKDESKH
DLIQELLCRIPGIRYVEEVEDRPYTDVHNIFEQALEAYRDEVAGKTFCVRVKRRGKHDFT
STEVERYVGGGLNQHIESAKVSLKDPQVTVKLEINDDKLVLVKSRLEGQGGYPIGTQEDV
LSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVKQVAHYLWNRFGSSHRVRFI
AVDFEPVVGEILEKVEDGQMGVVLKRMMVRAASSIAERYGVQALVTGEALGQVSSQTLTN
LRLIDNVSDTLILRPLISHDKEHIIKIAREIGTEDFAKTMPEYCGVISKSPTVKAVKAKI
EAEEAMFDFEVLDKVVSEARNVDIREIATQAAEVVPEVETVAEFVSTDVVLDVRAPDEVE
AQPLALENVEVKPLAFYKLATQFGDLDQNKTYLLYCDRGVMSRLQALYLKEQGFNNVKVY
RP