Protein Info for EX31_RS22075 in Rahnella sp. WP5

Annotation: gamma-glutamyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00066: gamma-glutamyltransferase
  • amino acids 30 to 541 (512 residues), 580.3 bits, E=1.6e-178
• PF01019: G_glu_transpept
  • amino acids 45 to 540 (496 residues), 582.9 bits, E=3.3e-179

Best Hits

Swiss-Prot: 59% identical to GGT_PSEUA: Glutathione hydrolase proenzyme (ggt) from Pseudomonas sp. (strain A14)

KEGG orthology group: K00681, gamma-glutamyltranspeptidase [EC: 2.3.2.2] (inferred from 100% identity to rah:Rahaq_3388)

MetaCyc: 50% identical to glutathione hydrolase proenzyme (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Gamma-glutamyltranspeptidase (EC 2.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Utilization of glutathione as a sulphur source (EC 2.3.2.2)

MetaCyc Pathways

• γ-glutamyl cycle (5/6 steps found)
• glutathione degradation (DUG pathway) (2/2 steps found)
• leukotriene biosynthesis (2/6 steps found)
• hypoglycin biosynthesis (4/14 steps found)
• peptido-conjugates in tissue regeneration biosynthesis (6/17 steps found)

KEGG Metabolic Maps

• Arachidonic acid metabolism
• Cyanoamino acid metabolism
• Glutathione metabolism
• Selenoamino acid metabolism
• Taurine and hypotaurine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.2.2

Use Curated BLAST to search for 2.3.2.2

Protein Sequence (583 amino acids)

>EX31_RS22075 gamma-glutamyltransferase (Rahnella sp. WP5)
MLRKSLIALAMLTSLPLYAASNPAVEAKNGMVVTSQYLASQVGVDILKMGGNAIDAAVAV
GYAQAVVNPCCGNIGGGGFMTVHLADGKDTFINFRETAPAAASANMYLDADGNVKKGASL
YGYLAAGVPGTVLGMDTVQKEYGKLTRQQVMAPAIKLARQGFVLTRADTDILDTTVKRFK
EDPEAARIFLRKDGSPLQPGDKLVQTDLANTLESISKHGPDAFYKGKIPAAVEAASKKGG
GILTAADFANYKVTEDAPIACSYRGYKFVSAPPPSSGGVTMCEILNIVEGYDLKSMGFNS
AASIHVLTEAMRHAYMDRNTYLGDPEFVKNPIDRLVSKSYAEEIRKKIEADKATPSTAVQ
PGMEPHEKPETTHYSIVDKMGNAVSTTYTVNGRFGSVVIAPGTGFFLNDEMDDFTTKVGE
KNLYGLVQGTKNAIAPGKRPLSSMSPSLVTKDGKIFMVLGSPGGSRIITITLQAALNVID
YGMAPQEAVDAPRIHHQWLPDEVYYEQRGVSQDSLNILQKMGYKMVEQTPWGATELIMVG
LPGAAGVTSANSGNDSAVSGKVREGYLYGANDVRRPAGAAIGY