Protein Info for EX31_RS21890 in Rahnella sp. WP5

Annotation: membrane-bound lytic murein transglycosylase MltF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 486 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF00497: SBP_bac_3" amino acids 46 to 267 (222 residues), 73.4 bits, see alignment E=1.5e-24 PF01464: SLT" amino acids 297 to 403 (107 residues), 83.4 bits, see alignment E=9.5e-28

Best Hits

Swiss-Prot: 69% identical to MLTF_YERE8: Membrane-bound lytic murein transglycosylase F (mltF) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: None (inferred from 100% identity to rah:Rahaq_3423)

MetaCyc: 61% identical to membrane-bound lytic murein transglycosylase F (Escherichia coli K-12 substr. MG1655)
4.2.2.f [EC: 4.2.2.f]

Predicted SEED Role

"Transglycosylase, Slt family"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.2.f

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (486 amino acids)

>EX31_RS21890 membrane-bound lytic murein transglycosylase MltF (Rahnella sp. WP5)
MTRLKINYVLMGIAALLLALALWPAVPWHRGEGDQLDQIKSRGELRVSTLNSPLTFMNSP
EGNLGFDYELAKRFADYLGVQLVVTQHTTIESMFDDLDHDNTDMLASGLVYNNERLKNYR
AGPEYYSVSQQLVYRQGSVRPKSFADIKGSLVVSAGSAHIATLEAASKKYPDLAWTTTDK
MSPRDLLQQVENGKLDYTLGDSVTIALMQRVYPRLAVAFDATDDEPVTWYYRKQPDDDSL
SAAMLDFFSQISEDGTLEKLEEKYLGHVGDFDYVDTRTFLNAIDSTLPDLQPLFEKYAGN
IDWRLLAAIAYQESHWDPQAKSPTGVRGVMMLTRATAGGLNVDDRTDAEQSIRGGAQYLS
HLMDKMPESVPQDERIWFALAAYNMGYGHMLDARKLTAQQKANPDSWADVKQRLPMLNQK
RFFSRTTYGYARGTQAYNYVENIRRYQLSLVGYLMEKEKQQAQKAALQAQLGQGYPVVNA
QEALAP