Protein Info for EX31_RS21425 in Rahnella sp. WP5

Annotation: beta-galactosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1032 PF02837: Glyco_hydro_2_N" amino acids 56 to 223 (168 residues), 220.2 bits, see alignment E=4.3e-69 PF00703: Glyco_hydro_2" amino acids 227 to 340 (114 residues), 49.3 bits, see alignment E=2e-16 PF02836: Glyco_hydro_2_C" amino acids 342 to 635 (294 residues), 389.4 bits, see alignment E=2.6e-120 PF16353: LacZ_4" amino acids 646 to 731 (86 residues), 70.5 bits, see alignment E=3e-23 PF02929: Bgal_small_N" amino acids 763 to 1028 (266 residues), 192.8 bits, see alignment E=2.1e-60

Best Hits

KEGG orthology group: K01190, beta-galactosidase [EC: 3.2.1.23] (inferred from 66% identity to eca:ECA1490)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1032 amino acids)

>EX31_RS21425 beta-galactosidase (Rahnella sp. WP5)
MNPSAEDKLSSLATVLARRDWEKPASTQCNRLPAHPPFNSWRNAEKARNNKASEKIRNLN
GEWAFSYFNRPEAVPEQWLQQDLPDADAVQVPSNWQLAGYDAPIYTNVQYPIPVNPPFVP
EENPAGCYSLTVSVDNDWLSSGQTRIVFNGVNSAFYLWCNGQWIGYSQDSRLPAEFDLSH
VLHAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVTLLHKPAEQITDLQVRTHLFHGF
TQAELEVQVSAAVPQDTAADYQVRVQLWQGDKPVAEHQQPLGSEIIDERGTAFDRTTLRL
PVSRPALWSAEQPALYRAVISLISPQGQLIEAEACDVGFRQVEISNGLLKVNGQPVLIRG
TNRHEHHPENGQVMDEATMRRDIVLMKQHNFNAVRCSHYPNHPLWYRLCDEYGLYVVDEA
NIETHGMQPMNRLSDDPVWFNAFSERVTRMVQRDRNHPCIIIWSLGNESGHGATHDALYR
WIKSVDPTRPVQYEGGGANSAATDIICPMYSRVEQDQPFPAVPKWSIKKWISMPDETRPL
ILCEYAHAMGNSFGGFDKYWKAFRQFPRLQGGFVWDWVDQSLLRTSENGERYMAYGGDFG
DTPNDRQFCMNGLVFADRTPHPSLFEAQRAQQFFQFSLTGTSPLMIEIQSEYLFRHSDNE
QFVWRIERDGEVISQGEAVLDIAPNGKQTLNLGDMPQAEGDLWLNVAVHQPQATAWSEAG
HRSAWDQWQLPQPLILPVPEKISAATPVLDQQGDVISVLHGAQCWQFSQQSGLLEQWFDG
KTPRLLSALRDQFVRAPVDNDIGVSEVTRIDPNAWVERWKAAGMYHLESRLLNISADQLQ
DGVVITTTHAFMAEEETRLLSRKIFRIDNQGELHISAEVRVASNVPSPGRIGLTCQLAET
AKNVSWLGLGPHENYPDRRLAAQHGRWNLPLTEMYTPYVFPTENGLRGETRELDYAGWTL
RGNFHFGLSRYSLQQLMDTSHRHMLREEEGTWLNIDGFHMGVGGDDSWSPSVSPDFLLTA
GQYHYSFTWKRA