Protein Info for EX31_RS21205 in Rahnella sp. WP5

Annotation: murein transglycosylase D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 462 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF01464: SLT" amino acids 112 to 226 (115 residues), 106.8 bits, see alignment E=5.3e-35 PF01476: LysM" amino acids 353 to 395 (43 residues), 48.7 bits, see alignment 6e-17 amino acids 412 to 452 (41 residues), 45.1 bits, see alignment 7.9e-16

Best Hits

Swiss-Prot: 65% identical to MLTD_ECOL6: Membrane-bound lytic murein transglycosylase D (mltD) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K08307, membrane-bound lytic murein transglycosylase D [EC: 3.2.1.-] (inferred from 100% identity to rah:Rahaq_0917)

MetaCyc: 65% identical to membrane-bound lytic murein transglycosylase D (Escherichia coli K-12 substr. MG1655)
4.2.2.f [EC: 4.2.2.f]; 4.2.2.f [EC: 4.2.2.f]

Predicted SEED Role

"Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-)" (EC 3.2.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.- or 4.2.2.f

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (462 amino acids)

>EX31_RS21205 murein transglycosylase D (Rahnella sp. WP5)
MKANAILIIASMLLTGCQASRQGVKVPEQHAQSLSSASQSEEAGQFTGDGRAASGGWLDG
NDTVSQQNLWNFIGDELKMKVPDNPRIREQRVKYLKNKSYLHDVTLRAEPYMYWITEQIK
KRNMPMELVLLPIVESAFDPKATSSANAAGLWQIVPQTGRNYGLKQNQWYDGRRDVVAST
TAALDMMQRLNKMFGGDWLLTVAAYNSGEGRVMQAVKANKARGRSTDFWALALPRETSIY
VPKMLALSDILKHSKKYGINLPKPSEDRALARVEVGQQMQLTQAAEMAGMSLTKLKSFNT
GYKKNVTAPNGPHYIVLPKAHADQLKDSLADGDIAAVQETKLASNTPSGAKQYKVRSGDS
LSGIAARLNVKTRDLQRWNNLRSAGALKVGQTLQVADNSVSNTSANGGSITYQVRKGDSL
SSIAKRHGVNIKDVMRWNTNADNLQPGNKLTLFVSNKMSPDT